Isolation and characterization of seventeen polymorphic microsatellite loci for the tropical sea star, Linckia multifora

被引:0
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作者
Benjamin J. Wainwright
Irma S. Arlyza
Stephen A. Karl
机构
[1] University of Hawai‘i,Hawai‘i Institute of Marine Biology
[2] Mānoa,undefined
[3] Research Center for Oceanography,undefined
[4] Indonesian Institute of Sciences,undefined
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Connectivity; Gene flow; Indonesia; Conservation;
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摘要
Seventeen polymorphic microsatellite loci were developed for the tropical sea star, Linckia multifora. Microsatellite enriched genomic libraries were constructed and sequenced using Roche 454 technology. The 17 loci were characterized in 24 individuals from the Kei islands, Indonesia. Observed levels of heterozygosity ranged from 0.29 to 1.00 with a mean of 6.0 alleles per locus. No pairs of loci showed evidence of significant linkage disequilibrium and four loci significantly deviated from Hardy–Weinberg expectations.
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页码:503 / 505
页数:2
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