Isolation and characterization of twenty-three polymorphic microsatellite loci for Linckia laevigata

被引：0
|
作者：
Wainwright, Benjamin J. [1 ]
Arlyza, Irma S. [2 ]
Karl, Stephen A. [1 ]
机构：
[1] Univ Hawaii, Hawaii Inst Marine Biol, Kaneohe, HI 96744 USA
[2] Indonesian Inst Sci, Res Ctr Oceanog, Jakarta 14430, Indonesia
来源：
CONSERVATION GENETICS RESOURCES | 2013年 / 5卷 / 01期
基金：
美国国家科学基金会;
关键词：
Connectivity; Gene flow; Indonesia; Conservation; MARINE PROTECTED AREAS; POPULATION-GENETICS; RESERVES; PRIMERS; IMPROVE; DESIGN; DNA;
D O I：
10.1007/s12686-012-9757-8
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Twenty-three polymorphic microsatellite loci were developed for the tropical sea star, Linckia laevigata. Microsatellite enriched genomic libraries were constructed and subsequently sequenced using Roche 454 technology. The 23 loci were characterized in 21 individuals from Pulau Derawan, Indonesia. Observed levels of heterozygosity ranged from 0.20 to 1.00 with a mean of 8.0 alleles per locus. No pairs of loci showed evidence of significant linkage disequilibrium and 4 loci significantly deviated from Hardy-Weinberg expectations.
引用
收藏
页码：157 / 159
页数：3
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