MicroRNA expression profiling on arrays enhanced with locked nucleic acids

被引：0
|
作者：
Roberts P. [1 ]
机构：
[1] Exiqon A/S, DK-2950 Vedbæk
来源：
Nature Methods | 2006年 / 3卷 / 4期
关键词：
D O I：
10.1038/nmeth869
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学科分类号：
摘要：
Here we describe a new array system (miRCURY™ LNA Array) for global microRNA (miRNA) profiling of biological samples. The miRCURY LNA Array system uses locked nucleic acid (LNA™), a high-affinity nucleotide analog, to enhance the array capture probes, giving them high sensitivity and excellent selectivity for miRNA targets. We have simultaneously developed a straightforward method for labeling miRNAs in a sample. As a result, the miRCURY LNA Array system can provide sensitiveand accurate profiling of miRNAs while requiring less sample and keeping sample handling stepsto a minimum. © 2006 Nature Publishing Group.
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页码：iii / iv
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