A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA)

被引：314
|
作者：
Castoldi, M
Schmidt, S
Benes, V
Noerholm, M
Kulozik, AE
Hentze, MW
Muckenthaler, MU
机构：
[1] Heidelberg Univ, Dept Pediat Oncol Hematol & Immunol, D-69120 Heidelberg, Germany
[2] EMBL, Genom Core Facil, Heidelberg, Germany
[3] EMBL, Gene Express Unit, Heidelberg, Germany
[4] EMBL, Mol Med Partnership Unit, Heidelberg, Germany
[5] Exiqon, Res & Dev, Vedbaek, Denmark
来源：
RNA | 2006年 / 12卷 / 05期
关键词：
microRNAs; microarrays; LNA; RNA translation; mouse;
D O I：
10.1261/rna.2332406
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
MicroRNAs represent a class of short (similar to 22 nt), noncoding regulatory RNAs involved in development, differentiation, and metabolism. We describe a novel microarray platform for genome-wide profiling of mature miRNAs (miChip) using locked nucleic acid (LNA)-modified capture probes. The biophysical properties of LNA were exploited to design probe sets for uniform, high-affinity hybridizations yielding highly accurate signals able to discriminate between single nucleotide differences and, hence, between closely related miRNA family members. The superior detection sensitivity eliminates the need for RNA size selection and/or amplification. MiChip will greatly simplify miRNA expression profiling of biological and clinical samples.
引用
收藏
页码：913 / 920
页数：8
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