MicroRNA expression profiling on arrays enhanced with locked nucleic acids

Here we describe a new array system (miRCURY™ LNA Array) for global microRNA (miRNA) profiling of biological samples. The miRCURY LNA Array system uses locked nucleic acid (LNA™), a high-affinity nucleotide analog, to enhance the array capture probes, giving them high sensitivity and excellent selectivity for miRNA targets. We have simultaneously developed a straightforward method for labeling miRNAs in a sample. As a result, the miRCURY LNA Array system can provide sensitiveand accurate profiling of miRNAs while requiring less sample and keeping sample handling stepsto a minimum. © 2006 Nature Publishing Group.