Real-Time Quantitative Fluorescent Reverse Transcriptase-PCR for Detection of Severe Acute Respiratory Syndrome-Associated Coronavirus RNA

被引:0
|
作者
Weijun Chen
Zuyuan Xu
Jingsong Mu
Bo He
Ling Yang
Lin Lin
Shufang Meng
Feng Mu
Haixue Gan
Shengyong Huang
Jie Wen
Jianqiu Fang
Jian Wang
机构
[1] Chinese Academy of Sciences,Beijing Genomics Institute
[2] Beijing BGI-GBI Biotech Co. Ltd,Institute of Genetics and Developmental Biology
[3] Chinese Academy of Sciences,undefined
[4] Beijing 302 Hospital,undefined
[5] National Institute for the Control of Pharmaceutical and Biological Products,undefined
关键词
Severe Acute Respiratory Syndrome; Severe Acute Respiratory Syndrome; Wide Linear Range; Atypical Pneumonia; Severe Acute Respiratory Syndrome Patient;
D O I
10.1007/BF03260067
中图分类号
学科分类号
摘要
Aim: SARS-associated coronavirus (SARS-CoV) has been confirmed as the pathogen for severe acute respiratory syndrome (SARS). The aim of our study was to construct a sensitive and specific real-time quantitative fluorescent (QF) reverse transcriptase (RT)-PCR method for the detection of SARS-CoV RNA.
引用
收藏
页码:231 / 235
页数:4
相关论文
共 50 条
  • [11] Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirus
    Bressler, AM
    Nolte, FS
    JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (03) : 987 - 991
  • [12] Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus
    Yu, Xin-Fen
    Pan, Jing-Cao
    Ye, Rong
    Xiang, Hai-Qing
    Kou, Yu
    Huang, Zhi-Cheng
    JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (03) : 837 - 841
  • [13] Detection of severe acute respiratory syndrome-associated coronavirus in pneumocytes of the lung
    Chow, KC
    Hsiao, CH
    Lin, TY
    Chen, CL
    Chiou, SH
    AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2004, 121 (04) : 574 - 580
  • [14] Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets
    Hu, WQ
    Bai, BK
    Hu, ZH
    Chen, Z
    An, XF
    Tang, LJ
    Yang, JH
    Wang, HL
    Wang, HZ
    JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (05) : 2041 - 2046
  • [15] Evaluation of advanced reverse transcription-PCR assays and an alternative PCR target region for detection of severe acute respiratory syndrome-associated coronavirus
    Drosten, C
    Chiu, LL
    Panning, M
    Leong, HN
    Preiser, W
    Tam, JS
    Günther, S
    Kramme, S
    Emmerich, P
    Ng, WL
    Schmitz, H
    Koay, ESC
    JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) : 2043 - 2047
  • [16] Detection of Infectious Bronchitis Virus with the Use of Real-Time Quantitative Reverse Transcriptase-PCR and Correlation with Virus Detection in Embryonated Eggs
    Roh, Ha-Jung
    Jordan, Brian J.
    Hilt, Deborah A.
    Jackwood, Mark W.
    AVIAN DISEASES, 2014, 58 (03) : 398 - 403
  • [17] Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA
    张仁礼
    郭中敏
    陆家海
    孟锦绣
    周灿权
    詹希美
    黄冰
    余新炳
    黄民
    潘兴华
    凌文华
    陈系古
    万卓越
    郑焕英
    鄢心革
    王一飞
    冉延超
    刘新健
    马俊鑫
    王承宇
    张必良
    Chinese Medical Journal, 2003, (08)
  • [18] Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA
    张仁礼
    郭中敏
    陆家海
    孟锦绣
    周灿权
    詹希美
    黄冰
    余新炳
    黄民
    潘兴华
    凌文华
    陈系古
    万卓越
    郑焕英
    鄢心革
    王一飞
    冉延超
    刘新健
    马俊鑫
    王承宇
    张必良
    中华医学杂志(英文版), 2003, (08) : 141 - 143
  • [19] Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA
    Zhang, RL
    Guo, ZM
    Lu, JH
    Meng, JX
    Zhou, CQ
    Zhan, XM
    Huang, B
    Yu, XB
    Huang, M
    Pan, XH
    Ling, WH
    Chen, XG
    Wan, ZY
    Zheng, HY
    Yan, XG
    Wang, YF
    Ran, YC
    Liu, XJ
    Ma, JX
    Wang, CY
    Zhang, BL
    CHINESE MEDICAL JOURNAL, 2003, 116 (08) : 1262 - 1264
  • [20] Severe acute respiratory syndrome-associated coronavirus infection
    Chan, PKS
    Ip, M
    Ng, KC
    Chan, RCW
    Wu, A
    Lee, N
    Rainer, TH
    Joynt, GM
    Sung, JJY
    Tam, JS
    EMERGING INFECTIOUS DISEASES, 2003, 9 (11) : 1453 - 1454