Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA

被引：0

作者：

Zhang, RL

Guo, ZM

Lu, JH ^{[1
]}

Meng, JX

Zhou, CQ

Zhan, XM

Huang, B

Yu, XB

Huang, M

Pan, XH

Ling, WH

Chen, XG

Wan, ZY

Zheng, HY

Yan, XG

Wang, YF

Ran, YC

Liu, XJ

Ma, JX

Wang, CY

Zhang, BL

机构：

[1] Sun Yat Sen Univ, Guangzhou 510089, Peoples R China

[2] Ctr Dis Control & Prevent Guangdon Province, Guangzhou 510300, Peoples R China

[3] Jinan Univ, Guangzhou 510632, Peoples R China

[4] Maxim Biotech Inc, San Francisco, CA 94080 USA

[5] Quartermaster Univ PLA, Changchun 130062, Peoples R China

[6] Univ Massachusetts, Sch Med, Worcester, MA 01605 USA

来源：

CHINESE MEDICAL JOURNAL | 2003年 / 116卷 / 08期

关键词：

small interfering RNA; severe acute respiratory syndrome; coronavirus; cytopathic effect; viral;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome ( SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases. Methods Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells. Results Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect. Conclusion siRNA may be effective in inhibiting SARS-associated coronavirus replication.

引用

页码：1262 / 1264

页数：3

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