Real-Time Quantitative Fluorescent Reverse Transcriptase-PCR for Detection of Severe Acute Respiratory Syndrome-Associated Coronavirus RNA

被引：0
|
作者：
Weijun Chen
Zuyuan Xu
Jingsong Mu
Bo He
Ling Yang
Lin Lin
Shufang Meng
Feng Mu
Haixue Gan
Shengyong Huang
Jie Wen
Jianqiu Fang
Jian Wang
机构：
[1] Chinese Academy of Sciences,Beijing Genomics Institute
[2] Beijing BGI-GBI Biotech Co. Ltd,Institute of Genetics and Developmental Biology
[3] Chinese Academy of Sciences,undefined
[4] Beijing 302 Hospital,undefined
[5] National Institute for the Control of Pharmaceutical and Biological Products,undefined
来源：
Molecular Diagnosis | 2004年 / 8卷 / 4期
关键词：
Severe Acute Respiratory Syndrome; Severe Acute Respiratory Syndrome; Wide Linear Range; Atypical Pneumonia; Severe Acute Respiratory Syndrome Patient;
D O I：
10.1007/BF03260067
中图分类号：
学科分类号：
摘要：
Aim: SARS-associated coronavirus (SARS-CoV) has been confirmed as the pathogen for severe acute respiratory syndrome (SARS). The aim of our study was to construct a sensitive and specific real-time quantitative fluorescent (QF) reverse transcriptase (RT)-PCR method for the detection of SARS-CoV RNA.
引用
收藏
页码：231 / 235
页数：4
相关论文
共 50 条
  • [41] Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR
    Ji Yeong Noh
    Sun-Woo Yoon
    Doo-Jin Kim
    Moo-Seung Lee
    Ji-Hyung Kim
    Woonsung Na
    Daesub Song
    Dae Gwin Jeong
    Hye Kwon Kim
    Archives of Virology, 2017, 162 : 1617 - 1623
  • [42] Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR
    Noh, Ji Yeong
    Yoon, Sun-Woo
    Kim, Doo-Jin
    Lee, Moo-Seung
    Kim, Ji-Hyung
    Na, Woonsung
    Song, Daesub
    Jeong, Dae Gwin
    Kim, Hye Kwon
    ARCHIVES OF VIROLOGY, 2017, 162 (06) : 1617 - 1623
  • [43] Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction
    Jiang, SS
    Chen, TC
    Yang, JY
    Hsiung, CA
    Su, IJ
    Liu, YL
    Chen, PC
    Juang, JL
    CLINICAL INFECTIOUS DISEASES, 2004, 38 (02) : 293 - 296
  • [44] Multiplex Real-Time Reverse Transcription PCR for Influenza A Virus, Influenza B Virus, and Severe Acute Respiratory Syndrome Coronavirus 2
    Shu, Bo
    Kirby, Marie K.
    Davis, William G.
    Warnes, Christine
    Liddell, Jimma
    Liu, Ji
    Wu, Kai-Hui
    Hassell, Norman
    Benitez, Alvaro J.
    Wilson, Malania M.
    Keller, Matthew W.
    Rambo-Martin, Benjamin L.
    Camara, Yamundow
    Winter, Jorn
    Kondor, Rebecca J.
    Zhou, Bin
    Spies, Stacey
    Rose, Laura E.
    Winchell, Jonas M.
    Limbago, Brandi M.
    Wentworth, David E.
    Barnes, John R.
    EMERGING INFECTIOUS DISEASES, 2021, 27 (07) : 1821 - 1830
  • [45] Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays
    Durand, Mathieu
    Thibault, Philippe
    Levesque, Simon
    Brault, Ariane
    Carignan, Alex
    Valiquette, Louis
    Martin, Philippe
    Labbe, Simon
    MICROBIAL CELL, 2022, 9 (01): : 1 - 20
  • [46] Severe acute respiratory syndrome-associated coronavirus genotype and its characterization
    李兰娟
    王志刚
    卢亦愚
    包其郁
    陈苏红
    吴南屏
    程苏云
    程苏云
    翁景清
    张严峻
    严菊英
    梅玲玲
    王晓萌
    朱函坪
    余迎朴
    张敏丽
    李敏红
    姚军
    陆群英
    姚苹苹
    伯晓晨
    沃健尔
    王升启
    胡松年
    ChineseMedicalJournal, 2003, (09)
  • [47] Enteric involvement of severe acute respiratory syndrome-associated coronavirus infection
    Leung, WK
    To, KF
    Chan, PKS
    Chan, HLY
    Wu, AKL
    Lee, N
    Yuen, KY
    Sung, JJY
    GASTROENTEROLOGY, 2003, 125 (04) : 1011 - 1017
  • [48] Severe acute respiratory syndrome-associated coronavirus genotype and its characterization
    李兰娟
    王志刚
    卢亦愚
    包其郁
    陈苏红
    吴南屏
    程苏云
    翁景清
    张严峻
    严菊英
    梅玲玲
    王晓萌
    朱函坪
    余迎朴
    张敏丽
    李敏红
    姚军
    陆群英
    姚苹苹
    伯晓晨
    沃健尔
    王升启
    胡松年
    中华医学杂志(英文版), 2003, (09) : 10 - 14
  • [49] Correction to: Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR
    Ji Yeong Noh
    Sun-Woo Yoon
    Doo-Jin Kim
    Moo-Seung Lee
    Ji-Hyung Kim
    Woonsung Na
    Daesub Song
    Dae Gwin Jeong
    Hye Kwon Kim
    Archives of Virology, 2018, 163 : 819 - 819
  • [50] Severe acute respiratory syndrome-associated coronavirus genotype and its characterization
    Li, LJ
    Wang, ZG
    Lu, YY
    Bao, QY
    Chen, SH
    Wu, NP
    Cheng, SY
    Weng, JQ
    Zhang, YJ
    Yan, JY
    Mei, LL
    Wang, XM
    Zhu, HP
    Yu, YP
    Zhang, ML
    Li, MH
    Yao, J
    Lu, QY
    Yao, PP
    Bo, XC
    Wo, JN
    Wang, SQ
    Hu, SN
    CHINESE MEDICAL JOURNAL, 2003, 116 (09) : 1288 - 1292
12345