Nano-mechanics of protein-based biostructures by atomic force microscopy

被引:0
|
作者
Ikai, A [1 ]
机构
[1] Tokyo Inst Technol, Biodynam Lab, Midori Ku, Yokohama, Kanagawa 2268501, Japan
关键词
protein stretching; nano-mechanics; nano-technology; atomic force Microscopy (AFM); extraction of membrane; protein;
D O I
10.1295/koron.61.595
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
I have studied the nano-mechanics of protein-based bio-structures to understand their fundamental properties that support the success of the life processes on this planet. I found that the intra-molecular structure of the globular protein is mechanically heterogeneous, with brittle local structures showing resistance against the tensile force of destruction. I also measured the force required to extract intrinsic membrane proteins from the live cell membrane and compared the measured force with that required for pore formation to the membrane. As the result, I propose a new method of harvesting membrane proteins from live cell membranes without destroying the organism.
引用
收藏
页码:595 / 600
页数:6
相关论文
共 50 条
  • [31] Atomic Force Microscopy (AFM) the Ultimate Nano Toolkit
    Gerber, Christoph
    SCIENCE OF ADVANCED MATERIALS, 2017, 9 (01) : 55 - 55
  • [32] Nano-steganography using atomic force microscopy
    Kudelka, Josef
    Martinek, Tomas
    Navratil, Milan
    Kresalek, Vojtech
    2016 IEEE 16TH INTERNATIONAL CONFERENCE ON NANOTECHNOLOGY (IEEE-NANO), 2016, : 157 - 159
  • [33] Nano mechanical processing of silicon by atomic force microscopy
    Miyake, S
    Wang, M
    Kim, J
    ADVANCES IN ABRASIVE TECHNOLOGY VIII, 2005, 291-292 : 401 - 406
  • [34] Atomic Force Microscopy as a Tool Applied to Nano/Biosensors
    Steffens, Clarice
    Leite, Fabio L.
    Bueno, Carolina C.
    Manzoli, Alexandra
    De Paula Herrmann, Paulo Sergio
    SENSORS, 2012, 12 (06) : 8278 - 8300
  • [35] Dynamic characterization of nano oscillators by atomic force microscopy
    Ilic, B.
    Krylov, S.
    Bellan, L. M.
    Craighead, H. G.
    PROCEEDINGS OF THE IEEE TWENTIETH ANNUAL INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, VOLS 1 AND 2, 2007, : 286 - +
  • [36] Classification of Dynamic Atomic Force Microscopy Control Modes Based on Asymptotic Nonlinear Mechanics
    Belikov, Sergey
    Magonov, Sergei
    2009 AMERICAN CONTROL CONFERENCE, VOLS 1-9, 2009, : 979 - +
  • [37] Mechanics and structure of titin oligomers explored with atomic force microscopy
    Kellermayer, MSZ
    Bustamante, C
    Granzier, HL
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2003, 1604 (02): : 105 - 114
  • [38] Nanoscale mechanics of brain abscess: An atomic force microscopy study
    Minelli, Eleonora
    Sassun, Tanya Enny
    Papi, Massimiliano
    Palmieri, Valentina
    Palermo, Francesca
    Perini, Giordano
    Antonelli, Manila
    Gianno, Francesca
    Maulucci, Giuseppe
    Ciasca, Gabriele
    De Spirito, Marco
    MICRON, 2018, 113 : 34 - 40
  • [39] Nanoscale mechanics by tomographic contact resonance atomic force microscopy
    Stan, Gheorghe
    Solares, Santiago D.
    Pittenger, Bede
    Erina, Natalia
    Su, Chanmin
    NANOSCALE, 2014, 6 (02) : 962 - 969
  • [40] Nanostructures and Mechanics of Living Exosomes Probed by Atomic Force Microscopy
    Li Mi
    Xu Xin-Ning
    Xi Ning
    Wang Wen-Xue
    Xing Xiao-Jing
    Liu Lian-Qing
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2021, 48 (01) : 100 - 110