Nano-mechanics of protein-based biostructures by atomic force microscopy

被引:0
|
作者
Ikai, A [1 ]
机构
[1] Tokyo Inst Technol, Biodynam Lab, Midori Ku, Yokohama, Kanagawa 2268501, Japan
关键词
protein stretching; nano-mechanics; nano-technology; atomic force Microscopy (AFM); extraction of membrane; protein;
D O I
10.1295/koron.61.595
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
I have studied the nano-mechanics of protein-based bio-structures to understand their fundamental properties that support the success of the life processes on this planet. I found that the intra-molecular structure of the globular protein is mechanically heterogeneous, with brittle local structures showing resistance against the tensile force of destruction. I also measured the force required to extract intrinsic membrane proteins from the live cell membrane and compared the measured force with that required for pore formation to the membrane. As the result, I propose a new method of harvesting membrane proteins from live cell membranes without destroying the organism.
引用
收藏
页码:595 / 600
页数:6
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