Biological influence of nanofibrous hydroxyapatite-polycaprolactone membrane on human periodontal ligament cell

Biological effect of the hydroxyapatite-coated nanofibrous membrane was mainly investigated on the proliferation and differentiation of human periodontal ligament fibroblast. SEM revealed favorable cell attachment and spreading appearance on I day and NITS assay showed increased cell proliferation on the hydroxyapatite-coated nanofiber membrane during 14 days. From 7 to 14 days, alkaline phosphatase activity of the hydroxyapatite-coated nanofiber was significantly increased when compared to that of control group. Hydroxyapatite-coated nanofibrous membrane showed prominent mineral formation on day 14. This study revealed that the developed hydroxyapatite-coated nanofibrous membrane had favorable effects on the proliferation and differentiation of human periodontal ligament fibroblast and might be a good candidate material for periodontal tissue regeneration.