X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels

被引：1
|
作者：
Chen, Qiang [1 ]
Xu, Yan [1 ]
Tang, Pei [1 ]
机构：
[1] Univ Pittsburgh, Sch Med, Pittsburgh, PA 15260 USA
来源：
CHEMICAL AND BIOCHEMICAL APPROACHES FOR THE STUDY OF ANESTHETIC FUNCTION, PT B | 2018年 / 603卷
关键词：
NICOTINIC ACETYLCHOLINE-RECEPTOR; CRYSTAL-STRUCTURES; TRANSMEMBRANE DOMAIN; STRUCTURAL BASIS; MECHANISM; CRYSTALLIZATION; PORE; NMR; INHIBITION; ISOFLURANE;
D O I：
10.1016/bs.mie.2018.01.017
中图分类号：
R614 [麻醉学];
学科分类号：
100217 ;
摘要：
X-ray crystallography is a powerful tool in structural biology and can offer insight into structured-based understanding of general anesthetic action on various relevant molecular targets, including pentameric ligand-gated ion channels (pLGICs). In this chapter, we outline the procedures for expression and purification of pLGICs. Optimization of crystallization conditions, especially to achieve high-resolution structures of pLGICs bound with general anesthetics, is also presented. Case studies of pLGICs bound with the volatile general anesthetic isoflurane, 2-bromoethanol, and the intravenous general anesthetic ketamine are revisited.
引用
收藏
页码：21 / 47
页数：27
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