X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels

被引：1
|
作者：
Chen, Qiang [1 ]
Xu, Yan [1 ]
Tang, Pei [1 ]
机构：
[1] Univ Pittsburgh, Sch Med, Pittsburgh, PA 15260 USA
来源：
CHEMICAL AND BIOCHEMICAL APPROACHES FOR THE STUDY OF ANESTHETIC FUNCTION, PT B | 2018年 / 603卷
关键词：
NICOTINIC ACETYLCHOLINE-RECEPTOR; CRYSTAL-STRUCTURES; TRANSMEMBRANE DOMAIN; STRUCTURAL BASIS; MECHANISM; CRYSTALLIZATION; PORE; NMR; INHIBITION; ISOFLURANE;
D O I：
10.1016/bs.mie.2018.01.017
中图分类号：
R614 [麻醉学];
学科分类号：
100217 ;
摘要：
X-ray crystallography is a powerful tool in structural biology and can offer insight into structured-based understanding of general anesthetic action on various relevant molecular targets, including pentameric ligand-gated ion channels (pLGICs). In this chapter, we outline the procedures for expression and purification of pLGICs. Optimization of crystallization conditions, especially to achieve high-resolution structures of pLGICs bound with general anesthetics, is also presented. Case studies of pLGICs bound with the volatile general anesthetic isoflurane, 2-bromoethanol, and the intravenous general anesthetic ketamine are revisited.
引用
收藏
页码：21 / 47
页数：27
相关论文
共 50 条
  • [21] Asymmetric Ligand Binding Facilitates Conformational Transitions in Pentameric Ligand-Gated Ion Channels
    Mowrey, David
    Cheng, Mary Hongying
    Liu, Lu Tian
    Willenbring, Dan
    Lu, Xinghua
    Wymore, Troy
    Xu, Yan
    Tang, Pei
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (06) : 2172 - 2180
  • [22] WHERE ARE THE BINDING SITES FOR ALCOHOLS AND ANESTHETICS ON CYS-LOOP LIGAND-GATED ION CHANNELS?
    Miller, K. W.
    ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2012, 36 : 362A - 362A
  • [23] Common binding sites for cholesterol and neurosteroids on a pentameric ligand-gated ion channel
    Budelier, Melissa M.
    Cheng, Wayland W. L.
    Chen, Zi-Wei
    Bracamontes, John R.
    Sugasawa, Yusuke
    Krishnan, Kathiresan
    Mydock-McGrane, Laurel
    Covey, Douglas F.
    Evers, Alex S.
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2019, 1864 (02): : 128 - 136
  • [24] ALCOHOL MODULATION VIA ALLOSTERIC TRANSMEMBRANE SITES IN PENTAMERIC LIGAND-GATED ION CHANNELS
    Howard, R. J.
    Heusser, S. A.
    Zhuang, Y.
    Lycksell, M.
    Klement, G.
    Orellana, L.
    Lindahl, E.
    ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2018, 42 : 60A - 60A
  • [25] Pentameric ligand-gated ion channels: insights from computation
    Salari, Reza
    Murlidaran, Sruthi
    Brannigan, Grace
    MOLECULAR SIMULATION, 2014, 40 (10-11) : 821 - 829
  • [26] Signal Transduction Pathways in the Pentameric Ligand-Gated Ion Channels
    Mowrey, David
    Chen, Qiang
    Liang, Yuhe
    Liang, Jie
    Xu, Yan
    Tang, Pei
    PLOS ONE, 2013, 8 (05):
  • [27] Structural Models of Ligand-Gated Ion Channels: Sites of Action for Anesthetics and Ethanol
    Olsen, Richard W.
    Li, Guo-Dong
    Wallner, Martin
    Trudell, James R.
    Bertaccini, Edward J.
    Lindahl, Erik
    Miller, Keith W.
    Alkana, Ronald L.
    Davies, Daryl L.
    ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2014, 38 (03) : 595 - 603
  • [28] Functional Evolution of Pentameric Ligand-Gated Ion Channels in Helminths
    Noonan, Jennifer
    Beech, Robin N.
    PROTEIN SCIENCE, 2017, 26 : 105 - 105
  • [29] Pentameric ligand-gated ion channels functioning at the atomic resolution
    Corringer, Pierre-Jean
    JOURNAL OF PHARMACOLOGICAL SCIENCES, 2016, 130 (03) : S2 - S2
  • [30] Insights into Intracellular Domains of Pentameric Ligand-Gated Ion Channels
    Jansen, Michaela
    BIOPHYSICAL JOURNAL, 2016, 110 (03) : 11A - 11A
12345