Adsorption of human serum albumin (HSA) on modified PET films monitored by QCM-D, XPS and AFM

被引：22
|
作者：
Indest, Tea [1 ]
Laine, Janne [2 ]
Kleinschek, Karin Stana [1 ]
Zemljic, Lidija Fras [1 ]
机构：
[1] Univ Maribor, Fac Mech Engn, Lab Characterizat & Proc Polymers, SI-2000 Maribor, Slovenia
[2] Helsinki Univ Technol, Lab Forest Prod Chem, FI-02015 Helsinki, Finland
来源：
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2010年 / 360卷 / 1-3期
关键词：
QCM-D; PET films; Human serum albumin (HSA); Chitosan sulfate; Fucoidan; QUARTZ-CRYSTAL MICROBALANCE; SURFACE-PLASMON RESONANCE; PROTEIN ADSORPTION; CHITOSAN; ELLIPSOMETRY; SPECTROSCOPY; KINETICS;
D O I：
10.1016/j.colsurfa.2010.03.003
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The adsorption behavior of human serum albumin (NSA) on differently modified poly(ethylene terephthalate) (PET) model film surfaces was studied using the quartz crystal microbalance (QCM). X-ray photoelectron spectroscopy (X PS) and atomic force microscopy (AFM) techniques The aim was to evaluate the influence of different modifications of PET surfaces with selected polysaccharides (chitosan, fucoidan, chitosan sulfate) on HSA adsorption. As a first step. PET hydrophilicity was increased by alkaline hydrolysis From these foils model PET films were prepared by the spin coating technique on a quartz crystal. Selected polysaccharides (chitosan, fucoidan and chitosan sulfate) were adsorbed from aqueous solutions on the PET surfaces Adsorption of NSA on the films was monitored with QCM-D The surface composition and morphology of the NSA covered PET films was analyzed using XPS and AFM It was found that due to steric repulsion the chitosan, fucoidan and chitosan sulfate adlayers reduced HSA adsorption, especially in case of chitosan/fucoidan and chitosan/chitosan sulfate covered films (C) 2010 Elsevier B V All rights reserved
引用
收藏
页码：210 / 219
页数：10
相关论文
共 50 条
  • [21] Adsorption behavior and cross-linking of EHEC and HM-EHEC at hydrophilic and hydrophobic modified surfaces monitored by SPR and QCM-D
    Hedin, Jesper
    Lofroth, Jan-Erik
    Nyden, Magnus
    LANGMUIR, 2007, 23 (11) : 6148 - 6155
  • [22] Adsorption of High-Molecular-Weight EOR Polymers on Glass Surfaces Using AFM and QCM-D
    Al-Hashmi, A. R.
    Luckham, P. F.
    Heng, J. Y. Y.
    Al-Maamari, R. S.
    Zaitoun, A.
    Al-Sharji, H. H.
    Al-Wehaibi, T. K.
    ENERGY & FUELS, 2013, 27 (05) : 2437 - 2444
  • [23] Adsorption and viscoelastic properties of fractionated mucin (BSM) and bovine serum albumin (BSA) studied with quartz crystal microbalance (QCM-D)
    Feiler, Adam A.
    Sahlholm, Anna
    Sandberg, Tomas
    Caldwell, Karin D.
    JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2007, 315 (02) : 475 - 481
  • [24] Interface potential sensing from adsorption of human serum albumin (HSA) on carbon nanotube (CNT) monitored by zero current potentiometry for HSA determination
    Wang, Huan
    Wu, Yi
    Song, Jun-Feng
    BIOSENSORS & BIOELECTRONICS, 2015, 72 : 225 - 229
  • [25] Crosslink Effect and Albumin Adsorption onto Chitosan/Alginate Multilayered Systems: An in situ QCM-D Study
    Martins, Gabriela V.
    Merino, Esther G.
    Mano, Joao F.
    Alves, Natalia M.
    MACROMOLECULAR BIOSCIENCE, 2010, 10 (12) : 1444 - 1455
  • [26] CELL 260-Top-down grafting of xyloglucan to gold monitored by QCM-D and AFM: Enzymatic activity and interactions with cellulose
    Nordgren, Niklas
    Eklof, Jens
    Zhou, Qi
    Brumer, Harry
    Rutland, Mark W.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2008, 235
  • [27] Study of the Human Albumin Role in the Formation of a Bacterial Biofilm on Urinary Devices Using QCM-D
    Garcia-Bonillo, Cristina
    Texido, Robert
    Reyes-Carmenaty, Guillermo
    Gilabert-Porres, Joan
    Borros, Salvador
    ACS APPLIED BIO MATERIALS, 2020, 3 (05) : 3354 - 3364
  • [28] FEASIBILITY OF RADIOLABELING FOR HUMAN-SERUM ALBUMIN (HSA) ADSORPTION STUDIES
    VANDERSCHEER, AT
    FEIJEN, J
    ELHORST, JK
    KRUGERSDAGNEAUX, PGLC
    SMOLDERS, CA
    JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1978, 66 (01) : 136 - 145
  • [29] Effect of grafted PEG chain conformation on albumin and lysozyme adsorption: A combined study using QCM-D and DPI
    Jin, Jing
    Han, Yuanyuan
    Zhang, Chang
    Liu, Jingchuan
    Jiang, Wei
    Yin, Jinghua
    Liang, Haojun
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2015, 136 : 838 - 844
  • [30] pH-dependent adsorption and conformational change of ferritin studied on metal oxide surfaces by a combination of QCM-D and AFM
    Hemmersam, Anne Gry
    Rechendorff, Kristian
    Besenbacher, Flemming
    Kasemo, Bengt
    Sutherland, Duncan S.
    JOURNAL OF PHYSICAL CHEMISTRY C, 2008, 112 (11): : 4180 - 4186
12345