Crosslink Effect and Albumin Adsorption onto Chitosan/Alginate Multilayered Systems: An in situ QCM-D Study

被引：61
|
作者：
Martins, Gabriela V. [1 ,2 ]
Merino, Esther G. [1 ,2 ]
Mano, Joao F. [1 ,2 ]
Alves, Natalia M. [1 ,2 ]
机构：
[1] Univ Minho, Dept Polymer Engn, Res Grp Biomat Biodegradables & Biomimet 3Bs, European Inst Excellence Tissue Engn & Regenerat, P-4806909 Caldas Das Taipas, Guimaraes, Portugal
[2] IBB Inst Biotechnol & Bioengn, PT Govt Associated Lab, Braga, Portugal
来源：
MACROMOLECULAR BIOSCIENCE | 2010年 / 10卷 / 12期
关键词：
biomaterials; crosslinking; layer-by-layer; protein adsorption; QCM-D; QUARTZ-CRYSTAL MICROBALANCE; SELF-ASSEMBLED FILMS; PROTEIN ADSORPTION; VISCOELASTIC PROPERTIES; HYDROPHOBIC SURFACES; ADHESION PROPERTIES; CELL-INTERACTIONS; GLOBULAR PROTEIN; HYALURONIC-ACID; SERUM-ALBUMIN;
D O I：
10.1002/mabi.201000193
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The adsorption of HSA onto CHI/ALG multilayer assemblies was assessed in situ using QCM-D. It was found that the behavior of HSA on biomaterials surface can be tuned by adjusting parameters of the polyelectrolyte system such as pH, layer number, crosslinker and polymer terminal layer. Our results confirmed the key role of electrostatic interactions during HSA adsorption, since oppositely charged surfaces were more effective in promoting protein adhesion. QCM-D data revealed that crosslinking (CHI/ALG)(5)CHI films allows HSA to become adsorbed in physiological conditions. Our results suggested that the biological potential of biopolymers and the mild conditions of the LbL technique turn these natural nanoassemblies into a suitable choice to be used as pH-sensitive coatings.
引用
收藏
页码：1444 / 1455
页数：12
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