Adsorption of human serum albumin (HSA) on modified PET films monitored by QCM-D, XPS and AFM

被引:22
|
作者
Indest, Tea [1 ]
Laine, Janne [2 ]
Kleinschek, Karin Stana [1 ]
Zemljic, Lidija Fras [1 ]
机构
[1] Univ Maribor, Fac Mech Engn, Lab Characterizat & Proc Polymers, SI-2000 Maribor, Slovenia
[2] Helsinki Univ Technol, Lab Forest Prod Chem, FI-02015 Helsinki, Finland
关键词
QCM-D; PET films; Human serum albumin (HSA); Chitosan sulfate; Fucoidan; QUARTZ-CRYSTAL MICROBALANCE; SURFACE-PLASMON RESONANCE; PROTEIN ADSORPTION; CHITOSAN; ELLIPSOMETRY; SPECTROSCOPY; KINETICS;
D O I
10.1016/j.colsurfa.2010.03.003
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The adsorption behavior of human serum albumin (NSA) on differently modified poly(ethylene terephthalate) (PET) model film surfaces was studied using the quartz crystal microbalance (QCM). X-ray photoelectron spectroscopy (X PS) and atomic force microscopy (AFM) techniques The aim was to evaluate the influence of different modifications of PET surfaces with selected polysaccharides (chitosan, fucoidan, chitosan sulfate) on HSA adsorption. As a first step. PET hydrophilicity was increased by alkaline hydrolysis From these foils model PET films were prepared by the spin coating technique on a quartz crystal. Selected polysaccharides (chitosan, fucoidan and chitosan sulfate) were adsorbed from aqueous solutions on the PET surfaces Adsorption of NSA on the films was monitored with QCM-D The surface composition and morphology of the NSA covered PET films was analyzed using XPS and AFM It was found that due to steric repulsion the chitosan, fucoidan and chitosan sulfate adlayers reduced HSA adsorption, especially in case of chitosan/fucoidan and chitosan/chitosan sulfate covered films (C) 2010 Elsevier B V All rights reserved
引用
收藏
页码:210 / 219
页数:10
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