Reagentless amperometric immunosensor for alpha-fetoprotein based on gold Nanoparticles/TiO2 colloids/prussian blue modified platinum electrode

被引：34
|
作者：
Yuan, Yanru [1 ]
Ynan, Ruo [1 ]
Chai, Yaqin [1 ]
Zhuo, Ying [1 ]
Shi, Yintao [1 ]
He, Xildan [1 ]
Miao, Xiangmin [1 ]
机构：
[1] SW Univ, Coll Chem & Chem Engn, Chongqing Key Lab Analyt Chem, Chongqing 400715, Peoples R China
来源：
ELECTROANALYSIS | 2007年 / 19卷 / 13期
关键词：
alpha-fetoprotein (AFP); amperometric immunosensor; gold nanoparticles; prussian blue; TiO2; colloids;
D O I：
10.1002/elan.200603868
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel reagentless amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was prepared by immobilizing TiO2 colloids on Prussian blue (PB) modified platinum electrode, which yielded a positively charged interface with strong adsorption to deposit gold nanoparticles for immobilization of alpha-fetoprotein antibody (anti-AFP). The factors influencing the performance of the proposed immunosensors were studied in detail. Under the optimized conditions, cyclic voltammograms determination of AFP showed a specific response in two concentration ranges from 3.0 to 30.0 ng/mL and from 30.0 to 300.0 ng/mL with a detection limit of 1.0 ng/mL at a signal-to-noise ratio of 3. The proposed immunosensor exhibited high selectivity, good reproducibility, long-term stability (> 2 months) and good repeatability.
引用
收藏
页码：1402 / 1410
页数：9
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