Reagentless amperometric immunosensor for alpha-fetoprotein based on gold Nanoparticles/TiO2 colloids/prussian blue modified platinum electrode

被引：34
|
作者：
Yuan, Yanru [1 ]
Ynan, Ruo [1 ]
Chai, Yaqin [1 ]
Zhuo, Ying [1 ]
Shi, Yintao [1 ]
He, Xildan [1 ]
Miao, Xiangmin [1 ]
机构：
[1] SW Univ, Coll Chem & Chem Engn, Chongqing Key Lab Analyt Chem, Chongqing 400715, Peoples R China
来源：
ELECTROANALYSIS | 2007年 / 19卷 / 13期
关键词：
alpha-fetoprotein (AFP); amperometric immunosensor; gold nanoparticles; prussian blue; TiO2; colloids;
D O I：
10.1002/elan.200603868
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel reagentless amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was prepared by immobilizing TiO2 colloids on Prussian blue (PB) modified platinum electrode, which yielded a positively charged interface with strong adsorption to deposit gold nanoparticles for immobilization of alpha-fetoprotein antibody (anti-AFP). The factors influencing the performance of the proposed immunosensors were studied in detail. Under the optimized conditions, cyclic voltammograms determination of AFP showed a specific response in two concentration ranges from 3.0 to 30.0 ng/mL and from 30.0 to 300.0 ng/mL with a detection limit of 1.0 ng/mL at a signal-to-noise ratio of 3. The proposed immunosensor exhibited high selectivity, good reproducibility, long-term stability (> 2 months) and good repeatability.
引用
收藏
页码：1402 / 1410
页数：9
相关论文
共 50 条
  • [21] Reagentless Amperometric Pyruvate Biosensor Based on a Prussian Blue- and Enzyme Nanoparticle-Modified Screen-Printed Carbon Electrode
    Thirumalai, Dinakaran
    Kim, Seonghye
    Kim, Suhkmann
    Chang, Seung-Cheol
    ACS OMEGA, 2020, 5 (46): : 30123 - 30129
  • [22] An amperometric immunosensor based on immobilization of hepatitis B surface antibody on gold electrode modified gold nanoparticles and horseradish peroxidase
    Zhuo, Y
    Yuan, R
    Chai, YQ
    Zhang, Y
    Li, XL
    Zhu, Q
    Wang, N
    ANALYTICA CHIMICA ACTA, 2005, 548 (1-2) : 205 - 210
  • [23] A micro amperometric immunosensor based on protein A/Gold nanoparticles/Self-assembled monolayer-modified gold electrode
    Xu, Yuanyuan
    Xia, Shanhong
    Bian, Chao
    Chen, Shaofeng
    2006 1ST IEEE INTERNATIONAL CONFERENCE ON NANO/MICRO ENGINEERED AND MOLECULAR SYSTEMS, VOLS 1-3, 2006, : 38 - 41
  • [24] Amperometric biosensors based on deposition of gold and platinum nanoparticles on polyvinylferrocene modified electrode for xanthine detection
    Bas, Salih Zeki
    Gulce, Handan
    Yildiz, Salih
    Gulce, Ahmet
    TALANTA, 2011, 87 : 189 - 196
  • [25] Development of Amperometric Glucose Biosensor Based on Prussian Blue Functionlized TiO2 Nanotube Arrays
    Gao, Zhi-Da
    Qu, Yongfang
    Li, Tongtong
    Shrestha, Nabeen K.
    Song, Yan-Yan
    SCIENTIFIC REPORTS, 2014, 4
  • [26] Development of Amperometric Glucose Biosensor Based on Prussian Blue Functionlized TiO2 Nanotube Arrays
    Zhi-Da Gao
    Yongfang Qu
    Tongtong Li
    Nabeen K. Shrestha
    Yan-Yan Song
    Scientific Reports, 4
  • [27] A new antibody immobilization strategy based on electro-deposition of gold nanoparticles and Prussian Blue for label-free amperometric immunosensor
    He, Xiulan
    Yuan, Ruo
    Chai, Yaqin
    Zhang, Ying
    Shi, Yintao
    BIOTECHNOLOGY LETTERS, 2007, 29 (01) : 149 - 155
  • [28] A new antibody immobilization strategy based on electro-deposition of gold nanoparticles and Prussian Blue for label-free amperometric immunosensor
    Xiulan He
    Ruo Yuan
    Yaqin Chai
    Ying Zhang
    Yintao Shi
    Biotechnology Letters, 2007, 29 : 149 - 155
  • [29] An amperometric biosensor for glucose based on self-assembling nanoparticles and electrosynthesis of poly-o-diaminobenzene on the Prussian blue-modified gold electrode
    Zhong, X
    Yuan, R
    Chai, YQ
    Dai, JY
    Liu, Y
    Tang, DP
    ANALYTICAL LETTERS, 2005, 38 (07) : 1085 - 1097
  • [30] A Novel H2O2 Amperometric Sensor based on Prussian Blue/Palladium Nanoparticles/Graphene Oxide Nanocomposite Modified Electrode
    Ahour, Fatemeh
    ANALYTICAL & BIOANALYTICAL ELECTROCHEMISTRY, 2019, 11 (06): : 812 - 829
12345