7-Deazaguanine modifications protect phage DNA from host restriction systems

被引：62

作者：

Hutinet, Geoffrey ^{[1
]}

Kot, Witold ^{[2
]}

Cui, Liang ^{[3
]}

Hillebrand, Roman ^{[4
,5
,15
]}

Balamkundu, Seetharamsingh ^{[3
]}

Gnanakalai, Shanmugavel ^{[3
]}

Neelakandan, Ramesh ^{[3
]}

Carstens, Alexander B. ^{[2
]}

Lui, Chuan Fa ^{[6
]}

Tremblay, Denise ^{[7
,8
,9
]}

Jacobs-Sera, Deborah ^{[10
,11
]}

Sassanfar, Mandana ^{[12
]}

Lee, Yan-Jiun ^{[13
]}

Weigele, Peter ^{[13
]}

Moineau, Sylvain ^{[7
,8
,9
]}

Hatfull, Graham F. ^{[10
,11
]}

Dedon, Peter C. ^{[3
,4
,5
]}

Hansen, Lars H. ^{[2
]}

de Crecy-Lagard, Valerie ^{[1
,14
]}

机构：

[1] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

[2] Aarhus Univ, Dept Environm Sci, Roskilde, Denmark

[3] Singapore MIT Alliance Res & Technol, Antimicrobial Resistance Interdisciplinary Res Gr, Campus Res Excellence & Technol Enterprise, Singapore 138602, Singapore

[4] MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[5] MIT, Ctr Environm Hlth Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[6] Nanyang Technol Univ, Sch Biol Sci, 60 Nanyang Dr, Singapore 637551, Singapore

[7] Univ Laval, Fac Sci & Genie, Dept Biochim Microbiol & Bioinformat, Quebec City, PQ G1V 0A6, Canada

[8] Univ Laval, Fac Med Dentaire, Felix Herelle Reference Ctr Bacterial Viruses, Quebec City, PQ G1V 0A6, Canada

[9] Univ Laval, Fac Med Dentaire, Grp Rech Ecol Buccale, Quebec City, PQ G1V 0A6, Canada

[10] Univ Pittsburgh, Pittsburgh Bacteriophage Inst, Pittsburgh, PA 15260 USA

[11] Univ Pittsburgh, Dept Biol Sci, Pittsburgh, PA 15260 USA

[12] MIT, Dept Biol, Cambridge, MA 02139 USA

[13] New England Biolabs Inc, Res Dept, Ipswich, MA 01938 USA

[14] Univ Florida, Genet Inst, Gainesville, FL 32610 USA

[15] Nitto Denko Avecia, 125 Fortune Blvd, Milford, MA 01757 USA

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

美国国家卫生研究院;

关键词：

DISCOVERY; BIOSYNTHESIS; MECHANISM; DATABASE; IDENTIFICATION; CYCLOHYDROLASE; GENERATION; SHARE; LIFE; WEB;

D O I：

10.1038/s41467-019-13384-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genome modifications are central components of the continuous arms race between viruses and their hosts. The archaeosine base (G(+)), which was thought to be found only in archaeal tRNAs, was recently detected in genomic DNA of Enterobacteria phage 9g and was proposed to protect phage DNA from a wide variety of restriction enzymes. In this study, we identify three additional 2'-deoxy-7-deazaguanine modifications, which are all intermediates of the same pathway, in viruses: 2'-deoxy-7-amido-7-deazaguanine (dADG), 2'-deoxy-7-cyano-7-deazaguanine (dPreQ(0)) and 2'-deoxy-7- aminomethyl-7-deazaguanine (dPreQ(1)). We identify 180 phages or archaeal viruses that encode at least one of the enzymes of this pathway with an overrepresentation (60%) of viruses potentially infecting pathogenic microbial hosts. Genetic studies with the Escherichia phage CAjan show that DpdA is essential to insert the 7-deazaguanine base in phage genomic DNA and that 2'-deoxy-7-deazaguanine modifications protect phage DNA from host restriction enzymes.

引用

页数：12

共 50 条

[41] A DNA methyltransferase can protect the genome from postdisturbance attack by a restriction-modification gene complex
Takahashi, N
Naito, Y
Handa, N
Kobayashi, I
JOURNAL OF BACTERIOLOGY, 2002, 184 (22) : 6100 - 6108
[42] CONVERSION OF THE FOKI ENDONUCLEASE TO A UNIVERSAL RESTRICTION ENZYME - CLEAVAGE OF PHAGE M13MP7 DNA AT PREDETERMINED SITES
PODHAJSKA, AJ
SZYBALSKI, W
GENE, 1985, 40 (2-3) : 175 - 182
[43] Enhanced Fusobacterium nucleatum Genetics Using Host DNA Methyltransferases To Bypass Restriction-Modification Systems
Umana, Ariana
Nguyen, Tam T. D.
Sanders, Blake E.
Williams, Kevin J.
Wozniak, Bryce
Slade, Daniel J.
JOURNAL OF BACTERIOLOGY, 2022, 204 (12)
[44] HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA-COLI .16. PHAGE LAMBDA-DNA CARRIES A SINGLE SITE OF AFFINITY FOR A-SPECIFIC RESTRICTION AND MODIFICATION
ARBER, W
WAUTERSW.D
KUHNLEIN, U
RIFAT, A
MOLECULAR AND GENERAL GENETICS, 1972, 115 (03): : 195 - +
[45] Peptides Derived from a Phage Display Library Inhibit Adhesion and Protect the Host against Infection by Paracoccidioides brasiliensis and Paracoccidioides lutzii
de Oliveira, Haroldo C.
Michaloski, Jussara S.
da Silva, Julhiany F.
Scorzoni, Liliana
de Paula e Silva, Ana C. A.
Marcos, Caroline M.
Assato, Patricia A.
Yamazaki, Danielle S.
Fusco-Almeida, Ana M.
Giordano, Ricardo J.
Mendes-Giannini, Maria J. S.
FRONTIERS IN PHARMACOLOGY, 2016, 7
[46] Restriction endonuclease cleavage of phage DNA enables resuscitation from Cas13-induced bacterial dormancy
Williams, Madison C. C.
Reker, Alexandra E. E.
Margolis, Shally R. R.
Liao, Jingqiu
Wiedmann, Martin
Rojas, Enrique R. R.
Meeske, Alexander J. J.
NATURE MICROBIOLOGY, 2023, 8 (03) : 400 - +
[47] EFFECT OF DIFFERENTIAL METHYLATION BY ESCHERICHIA-COLI OF PLASMID DNA AND PHAGE-T7 AND LAMBDA-DNA ON THE CLEAVAGE BY RESTRICTION ENDONUCLEASE-MBOI FROM MORAXELLA-BOVIS
DREISEIKELMANN, B
EICHENLAUB, R
WACKERNAGEL, W
BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 562 (03) : 418 - 428
[48] Restriction endonuclease cleavage of phage DNA enables resuscitation from Cas13-induced bacterial dormancy
Madison C. Williams
Alexandra E. Reker
Shally R. Margolis
Jingqiu Liao
Martin Wiedmann
Enrique R. Rojas
Alexander J. Meeske
Nature Microbiology, 2023, 8 : 400 - 409
[49] Isolation and characterization of marine psychrophilic phage-host systems from Arctic sea ice
Borriss, M
Helmke, E
Hanschke, R
Schweder, T
EXTREMOPHILES, 2003, 7 (05) : 377 - 384
[50] DNA TRANSFER FROM PHAGE T5 TO HOST CELLS - DEPENDENCE ON INTERCURRENT PROTEIN SYNTHESIS
LANNI, YT
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1965, 53 (05) : 969 - &

← 1 2 3 4 5 →