7-Deazaguanine modifications protect phage DNA from host restriction systems

被引：62

作者：

Hutinet, Geoffrey ^{[1
]}

Kot, Witold ^{[2
]}

Cui, Liang ^{[3
]}

Hillebrand, Roman ^{[4
,5
,15
]}

Balamkundu, Seetharamsingh ^{[3
]}

Gnanakalai, Shanmugavel ^{[3
]}

Neelakandan, Ramesh ^{[3
]}

Carstens, Alexander B. ^{[2
]}

Lui, Chuan Fa ^{[6
]}

Tremblay, Denise ^{[7
,8
,9
]}

Jacobs-Sera, Deborah ^{[10
,11
]}

Sassanfar, Mandana ^{[12
]}

Lee, Yan-Jiun ^{[13
]}

Weigele, Peter ^{[13
]}

Moineau, Sylvain ^{[7
,8
,9
]}

Hatfull, Graham F. ^{[10
,11
]}

Dedon, Peter C. ^{[3
,4
,5
]}

Hansen, Lars H. ^{[2
]}

de Crecy-Lagard, Valerie ^{[1
,14
]}

机构：

[1] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

[2] Aarhus Univ, Dept Environm Sci, Roskilde, Denmark

[3] Singapore MIT Alliance Res & Technol, Antimicrobial Resistance Interdisciplinary Res Gr, Campus Res Excellence & Technol Enterprise, Singapore 138602, Singapore

[4] MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[5] MIT, Ctr Environm Hlth Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[6] Nanyang Technol Univ, Sch Biol Sci, 60 Nanyang Dr, Singapore 637551, Singapore

[7] Univ Laval, Fac Sci & Genie, Dept Biochim Microbiol & Bioinformat, Quebec City, PQ G1V 0A6, Canada

[8] Univ Laval, Fac Med Dentaire, Felix Herelle Reference Ctr Bacterial Viruses, Quebec City, PQ G1V 0A6, Canada

[9] Univ Laval, Fac Med Dentaire, Grp Rech Ecol Buccale, Quebec City, PQ G1V 0A6, Canada

[10] Univ Pittsburgh, Pittsburgh Bacteriophage Inst, Pittsburgh, PA 15260 USA

[11] Univ Pittsburgh, Dept Biol Sci, Pittsburgh, PA 15260 USA

[12] MIT, Dept Biol, Cambridge, MA 02139 USA

[13] New England Biolabs Inc, Res Dept, Ipswich, MA 01938 USA

[14] Univ Florida, Genet Inst, Gainesville, FL 32610 USA

[15] Nitto Denko Avecia, 125 Fortune Blvd, Milford, MA 01757 USA

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

美国国家卫生研究院;

关键词：

DISCOVERY; BIOSYNTHESIS; MECHANISM; DATABASE; IDENTIFICATION; CYCLOHYDROLASE; GENERATION; SHARE; LIFE; WEB;

D O I：

10.1038/s41467-019-13384-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genome modifications are central components of the continuous arms race between viruses and their hosts. The archaeosine base (G(+)), which was thought to be found only in archaeal tRNAs, was recently detected in genomic DNA of Enterobacteria phage 9g and was proposed to protect phage DNA from a wide variety of restriction enzymes. In this study, we identify three additional 2'-deoxy-7-deazaguanine modifications, which are all intermediates of the same pathway, in viruses: 2'-deoxy-7-amido-7-deazaguanine (dADG), 2'-deoxy-7-cyano-7-deazaguanine (dPreQ(0)) and 2'-deoxy-7- aminomethyl-7-deazaguanine (dPreQ(1)). We identify 180 phages or archaeal viruses that encode at least one of the enzymes of this pathway with an overrepresentation (60%) of viruses potentially infecting pathogenic microbial hosts. Genetic studies with the Escherichia phage CAjan show that DpdA is essential to insert the 7-deazaguanine base in phage genomic DNA and that 2'-deoxy-7-deazaguanine modifications protect phage DNA from host restriction enzymes.

引用

页数：12

共 50 条

[11] Bypass of DNA-Protein Cross-links Conjugated to the 7-Deazaguanine Position of DNA by Translesion Synthesis Polymerases
Wickramaratne, Susith
Ji, Shaofei
Mukherjee, Shivam
Su, Yan
Pence, Matthew G.
Lior-Hoffmann, Lee
Fu, Iwen
Broyde, Suse
Guengerich, F. Peter
Distefano, Mark
Scharer, Orlando D.
Sham, Yuk Yin
Tretyakova, Natalia
JOURNAL OF BIOLOGICAL CHEMISTRY, 2016, 291 (45) : 23589 - 23603
[12] Oligonucleotides containing 7-deazaguanine: The role of 7- and 8-methyl groups during the B-Z transition of DNA
Seela, F
Chen, YM
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 1996, 61 : S112 - S113
[13] Pyrene and bis-pyrene DNA nucleobase conjugates: excimer and monomer fluorescence of linear and dendronized cytosine and 7-deazaguanine click adducts
Mei, Hui
Ingale, Sachin A.
Seela, Frank
TETRAHEDRON, 2013, 69 (23) : 4731 - 4742
[14] "Double Click" Reaction on 7-Deazaguanine DNA: Synthesis and Excimer Fluorescence of Nucleosides and Oligonucleotides with Branched Side Chains Decorated with Proximal Pyrenes
Seela, Frank
Ingale, Sachin A.
JOURNAL OF ORGANIC CHEMISTRY, 2010, 75 (02): : 284 - 295
[15] Silver(I)-Mediated Base Pairs in DNA Sequences Containing 7-Deazaguanine/Cytosine: towards DNA with Entirely Metallated Watson-Crick Base Pairs
Mendez-Arriaga, Jose M.
Maldonado, Carmen R.
Dobado, Jose A.
Galindo, Miguel A.
CHEMISTRY-A EUROPEAN JOURNAL, 2018, 24 (18) : 4583 - 4589
[16] ENTRY OF BACTERIOPHAGE T7 DNA INTO THE CELL AND ESCAPE FROM HOST RESTRICTION
MOFFATT, BA
STUDIER, FW
JOURNAL OF BACTERIOLOGY, 1988, 170 (05) : 2095 - 2105
[17] DNA Polymerase-Mediated Synthesis of Unbiased Threose Nucleic Acid (TNA) Polymers Requires 7-Deazaguanine To Suppress G:G Mispairing during TNA Transcription
Dunn, Matthew R.
Larsen, Andrew C.
Zahurancik, Walter J.
Fahmi, Nour Eddine
Meyers, Madeline
Suo, Zucai
Chaput, John C.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (12) : 4014 - 4017
[18] HOST SPECIFICITY OF DNA IN HEMOPHILUS-INFLUENZAE - INVIVO ACTION OF RESTRICTION ENDONUCLEASES ON PHAGE AND BACTERIAL-DNA
PIEKAROWICZ, A
BRZEZINSKI, R
KAUC, L
ACTA MICROBIOLOGICA POLONICA SERIES A-MICROBIOLOGIA GENERALIS, 1975, 7 (02): : 51 - 65
[19] SENSITIVITY OF PHAGE DNA AND PLASMID DNA FOR A RESTRICTION ENZYME FROM STAPHYLOCOCCUS-AUREUS
STOBBERINGH, EE
MEIJERS, JA
VANKATSRENAUD, JH
ANTONIE VAN LEEUWENHOEK JOURNAL OF MICROBIOLOGY, 1979, 45 (01): : 19 - 23
[20] RESTRICTION INVIVO .4. EFFECT OF RESTRICTION OF PARENTAL DNA ON THE EXPRESSION OF RESTRICTION ALLEVIATION SYSTEMS IN PHAGE-T4
DHARMALINGAM, K
GOLDBERG, EB
VIROLOGY, 1979, 96 (02) : 404 - 411

← 1 2 3 4 5 →