7-Deazaguanine modifications protect phage DNA from host restriction systems

被引：62

作者：

Hutinet, Geoffrey ^{[1
]}

Kot, Witold ^{[2
]}

Cui, Liang ^{[3
]}

Hillebrand, Roman ^{[4
,5
,15
]}

Balamkundu, Seetharamsingh ^{[3
]}

Gnanakalai, Shanmugavel ^{[3
]}

Neelakandan, Ramesh ^{[3
]}

Carstens, Alexander B. ^{[2
]}

Lui, Chuan Fa ^{[6
]}

Tremblay, Denise ^{[7
,8
,9
]}

Jacobs-Sera, Deborah ^{[10
,11
]}

Sassanfar, Mandana ^{[12
]}

Lee, Yan-Jiun ^{[13
]}

Weigele, Peter ^{[13
]}

Moineau, Sylvain ^{[7
,8
,9
]}

Hatfull, Graham F. ^{[10
,11
]}

Dedon, Peter C. ^{[3
,4
,5
]}

Hansen, Lars H. ^{[2
]}

de Crecy-Lagard, Valerie ^{[1
,14
]}

机构：

[1] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

[2] Aarhus Univ, Dept Environm Sci, Roskilde, Denmark

[3] Singapore MIT Alliance Res & Technol, Antimicrobial Resistance Interdisciplinary Res Gr, Campus Res Excellence & Technol Enterprise, Singapore 138602, Singapore

[4] MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[5] MIT, Ctr Environm Hlth Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[6] Nanyang Technol Univ, Sch Biol Sci, 60 Nanyang Dr, Singapore 637551, Singapore

[7] Univ Laval, Fac Sci & Genie, Dept Biochim Microbiol & Bioinformat, Quebec City, PQ G1V 0A6, Canada

[8] Univ Laval, Fac Med Dentaire, Felix Herelle Reference Ctr Bacterial Viruses, Quebec City, PQ G1V 0A6, Canada

[9] Univ Laval, Fac Med Dentaire, Grp Rech Ecol Buccale, Quebec City, PQ G1V 0A6, Canada

[10] Univ Pittsburgh, Pittsburgh Bacteriophage Inst, Pittsburgh, PA 15260 USA

[11] Univ Pittsburgh, Dept Biol Sci, Pittsburgh, PA 15260 USA

[12] MIT, Dept Biol, Cambridge, MA 02139 USA

[13] New England Biolabs Inc, Res Dept, Ipswich, MA 01938 USA

[14] Univ Florida, Genet Inst, Gainesville, FL 32610 USA

[15] Nitto Denko Avecia, 125 Fortune Blvd, Milford, MA 01757 USA

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

美国国家卫生研究院;

关键词：

DISCOVERY; BIOSYNTHESIS; MECHANISM; DATABASE; IDENTIFICATION; CYCLOHYDROLASE; GENERATION; SHARE; LIFE; WEB;

D O I：

10.1038/s41467-019-13384-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genome modifications are central components of the continuous arms race between viruses and their hosts. The archaeosine base (G(+)), which was thought to be found only in archaeal tRNAs, was recently detected in genomic DNA of Enterobacteria phage 9g and was proposed to protect phage DNA from a wide variety of restriction enzymes. In this study, we identify three additional 2'-deoxy-7-deazaguanine modifications, which are all intermediates of the same pathway, in viruses: 2'-deoxy-7-amido-7-deazaguanine (dADG), 2'-deoxy-7-cyano-7-deazaguanine (dPreQ(0)) and 2'-deoxy-7- aminomethyl-7-deazaguanine (dPreQ(1)). We identify 180 phages or archaeal viruses that encode at least one of the enzymes of this pathway with an overrepresentation (60%) of viruses potentially infecting pathogenic microbial hosts. Genetic studies with the Escherichia phage CAjan show that DpdA is essential to insert the 7-deazaguanine base in phage genomic DNA and that 2'-deoxy-7-deazaguanine modifications protect phage DNA from host restriction enzymes.

引用

页数：12

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