7-Deazaguanine modifications protect phage DNA from host restriction systems

被引：62

作者：

Hutinet, Geoffrey ^{[1
]}

Kot, Witold ^{[2
]}

Cui, Liang ^{[3
]}

Hillebrand, Roman ^{[4
,5
,15
]}

Balamkundu, Seetharamsingh ^{[3
]}

Gnanakalai, Shanmugavel ^{[3
]}

Neelakandan, Ramesh ^{[3
]}

Carstens, Alexander B. ^{[2
]}

Lui, Chuan Fa ^{[6
]}

Tremblay, Denise ^{[7
,8
,9
]}

Jacobs-Sera, Deborah ^{[10
,11
]}

Sassanfar, Mandana ^{[12
]}

Lee, Yan-Jiun ^{[13
]}

Weigele, Peter ^{[13
]}

Moineau, Sylvain ^{[7
,8
,9
]}

Hatfull, Graham F. ^{[10
,11
]}

Dedon, Peter C. ^{[3
,4
,5
]}

Hansen, Lars H. ^{[2
]}

de Crecy-Lagard, Valerie ^{[1
,14
]}

机构：

[1] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

[2] Aarhus Univ, Dept Environm Sci, Roskilde, Denmark

[3] Singapore MIT Alliance Res & Technol, Antimicrobial Resistance Interdisciplinary Res Gr, Campus Res Excellence & Technol Enterprise, Singapore 138602, Singapore

[4] MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[5] MIT, Ctr Environm Hlth Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[6] Nanyang Technol Univ, Sch Biol Sci, 60 Nanyang Dr, Singapore 637551, Singapore

[7] Univ Laval, Fac Sci & Genie, Dept Biochim Microbiol & Bioinformat, Quebec City, PQ G1V 0A6, Canada

[8] Univ Laval, Fac Med Dentaire, Felix Herelle Reference Ctr Bacterial Viruses, Quebec City, PQ G1V 0A6, Canada

[9] Univ Laval, Fac Med Dentaire, Grp Rech Ecol Buccale, Quebec City, PQ G1V 0A6, Canada

[10] Univ Pittsburgh, Pittsburgh Bacteriophage Inst, Pittsburgh, PA 15260 USA

[11] Univ Pittsburgh, Dept Biol Sci, Pittsburgh, PA 15260 USA

[12] MIT, Dept Biol, Cambridge, MA 02139 USA

[13] New England Biolabs Inc, Res Dept, Ipswich, MA 01938 USA

[14] Univ Florida, Genet Inst, Gainesville, FL 32610 USA

[15] Nitto Denko Avecia, 125 Fortune Blvd, Milford, MA 01757 USA

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

美国国家卫生研究院;

关键词：

DISCOVERY; BIOSYNTHESIS; MECHANISM; DATABASE; IDENTIFICATION; CYCLOHYDROLASE; GENERATION; SHARE; LIFE; WEB;

D O I：

10.1038/s41467-019-13384-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genome modifications are central components of the continuous arms race between viruses and their hosts. The archaeosine base (G(+)), which was thought to be found only in archaeal tRNAs, was recently detected in genomic DNA of Enterobacteria phage 9g and was proposed to protect phage DNA from a wide variety of restriction enzymes. In this study, we identify three additional 2'-deoxy-7-deazaguanine modifications, which are all intermediates of the same pathway, in viruses: 2'-deoxy-7-amido-7-deazaguanine (dADG), 2'-deoxy-7-cyano-7-deazaguanine (dPreQ(0)) and 2'-deoxy-7- aminomethyl-7-deazaguanine (dPreQ(1)). We identify 180 phages or archaeal viruses that encode at least one of the enzymes of this pathway with an overrepresentation (60%) of viruses potentially infecting pathogenic microbial hosts. Genetic studies with the Escherichia phage CAjan show that DpdA is essential to insert the 7-deazaguanine base in phage genomic DNA and that 2'-deoxy-7-deazaguanine modifications protect phage DNA from host restriction enzymes.

引用

页数：12

共 50 条

[1] 7-Deazaguanine modifications protect phage DNA from host restriction systems
Geoffrey Hutinet
Witold Kot
Liang Cui
Roman Hillebrand
Seetharamsingh Balamkundu
Shanmugavel Gnanakalai
Ramesh Neelakandan
Alexander B. Carstens
Chuan Fa Lui
Denise Tremblay
Deborah Jacobs-Sera
Mandana Sassanfar
Yan-Jiun Lee
Peter Weigele
Sylvain Moineau
Graham F. Hatfull
Peter C. Dedon
Lars H. Hansen
Valérie de Crécy-Lagard
Nature Communications, 10
[2] Structural Basis of 7-Deazaguanine Modification of DNA in Bacteria and Phage
Hellie, Eric
Alkuzweny, Sana
Bayooz, Susan
Phuc Phan
Savage, Joshua
Luthra, Amit
Iwata-Reuyl, Dirk
Swairjo, Manal
FASEB JOURNAL, 2020, 34
[3] Polymerase Synthesis and Restriction Enzyme Cleavage of DNA Containing 7-Substituted 7-Deazaguanine Nucleobases
Mackova, Michaela
Bohacova, Sona
Perlikova, Pavla
Slavetinska, Lenka Postova
Hocek, Michal
CHEMBIOCHEM, 2015, 16 (15) : 2225 - 2236
[4] Dynamics and energetics of hole trapping in DNA by 7-deazaguanine
Lewis, FD
Liu, JQ
Liu, XY
Zuo, XB
Hayes, RT
Wasielewski, MR
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2002, 41 (06) : 1026 - +
[5] Novel genomic island modifies DNA with 7-deazaguanine derivatives
Thiaville, Jennifer J.
Kellner, Stefanie M.
Yuan, Yifeng
Hutinet, Geoffrey
Thiaville, Patrick C.
Jumpathong, Watthanachai
Mohapatra, Susovan
Brochier-Armanet, Celine
Letarov, Andrey V.
Hillebrand, Roman
Malik, Chanchal K.
Rizzo, Carmelo J.
Dedon, Peter C.
de Crecy-Lagard, Valerie
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (11) : E1452 - E1459
[6] 7-deazaguanine DNA: Oligonucleotides with hydrophobic or cationic side chains
Ramzaeva, N
Mittelbach, C
Seela, F
HELVETICA CHIMICA ACTA, 1997, 80 (06) : 1809 - 1822
[7] Electron transfer from 7-deazaguanine to ruthenium polypyridyl complexes.
Yang, IV
Thorp, HH
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2000, 220 : U467 - U467
[8] DNA-mediated charge transfer between 7-deazaguanine and a ruthenium(II) metallointercalator.
Treadway, CR
Manger, MM
Barton, JK
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2000, 219 : U871 - U871
[9] ETHIDIUM-BROMIDE DOES NOT FLUORESCE WHEN INTERCALATED ADJACENT TO 7-DEAZAGUANINE IN DUPLEX DNA
LATIMER, LJP
LEE, JS
JOURNAL OF BIOLOGICAL CHEMISTRY, 1991, 266 (21) : 13849 - 13851
[10] Utilization of Electrochemical Activity of 7-Deazaguanine in the Monitoring of PCR Amplification of G-rich DNA Strands
Fojtova, Miloslava
Horakova, Petra
Pivonkova, Hana
Vychodilova, Zdenka
Fojta, Miroslav
MODERN ELECTROCHEMICAL METHODS XXX, 2010, : 50 - 54

← 1 2 3 4 5 →