A novel DNA binding protein-based platform for electrochemical detection of miRNA

被引：7
|
作者：
Umer, Muhammad [1 ]
Aziz, Nahian B. [2 ]
Mahmudunnabi, Rabbee G. [3 ,4 ]
Shim, Yoon-Bo [3 ,4 ]
Salomon, Carlos [2 ]
Shiddiky, Muhammad J. A. [1 ,5 ]
机构：
[1] Griffith Univ, Queensland Micro & Nanotechnol Ctr QMNC, Nathan, Qld 4111, Australia
[2] Univ Queensland, Royal Brisbane & Womens Hosp, Ctr Clin Res, Exosome Biol Lab,Ctr Clin Diagnost, Brisbane, Qld, Australia
[3] Pusan Natl Univ, Dept Chem, Busan 46241, South Korea
[4] Pusan Natl Univ, Inst BioPhysio Sensor Technol, Busan 46241, South Korea
[5] Griffith Univ, Sch Environm & Sci, Nathan, Qld 4111, Australia
来源：
ANALYST | 2021年 / 146卷 / 18期
基金：
新加坡国家研究基金会; 英国医学研究理事会; 澳大利亚研究理事会;
关键词：
AMPLIFICATION; REPLICATION; POLYMERASE;
D O I：
10.1039/d1an00935d
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We present a novel amplification-free sandwich type platform assay for electrochemical detection of miRNA. The assay is based on T4 DNA polymerase mediated synthesis of the p53 binding DNA sequence at the 3 ' end of target miRNA. The resulting miRNA-DNA chimera is detected via an electrochemical sandwich hybridization assay where HRP-labelled p53 binds to its recognition sequence and an amperometric signal is generated by hydroquinone-mediated enzymatic reduction of H2O2. The limit of detection of our assay was estimated to be 22 fM with a linear dynamic range of 100 fM-1 nM. This new platform method of detecting miRNA shows superior performance to conventional electrochemical miRNA biosensors and has the potential for amplification-free analysis of miRNA with high specificity and sensitivity.
引用
收藏
页码：5496 / +
页数：7
相关论文
共 50 条
  • [1] A Protein-Based Electrochemical Biosensor Array Platform for Integrated Microsystems
    Huang, Yue
    Liu, Ying
    Hassler, Brian L.
    Worden, R. Mark
    Mason, Andrew J.
    IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, 2013, 7 (01) : 43 - 51
  • [2] A novel zinc finger protein-based amperometric biosensor for miRNA determination
    Povedano, Eloy
    Ruiz-Valdepenas Montiel, Victor
    Gamella, Maria
    Serafin, Veronica
    Pedrero, Maria
    Moranova, Ludmila
    Bartosik, Martin
    Montoya, Juan Jose
    Yanez-Sedeno, Paloma
    Campuzano, Susana
    Pingarron, Jose M.
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2020, 412 (21) : 5031 - 5041
  • [3] PROTEIN-BASED ELECTROCHEMICAL BIOSENSOR FOR DETECTION OF SILVER(I) IONS
    Krizkova, Sona
    Huska, Dalibor
    Beklova, Miroslava
    Hubalek, Jaromir
    Adam, Vojtech
    Trnkova, Libuse
    Kizek, Rene
    ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2010, 29 (03) : 492 - 496
  • [4] A novel electrochemical aptasensor for ultrasensitive detection of fluoroquinolones based on single-stranded DNA-binding protein
    Abnous, Khalil
    Danesh, Noor Mohammad
    Alibolandi, Mona
    Ramezani, Mohammad
    Taghdisi, Seyed Mohammad
    Emrani, Ahmad Sarreshtehdar
    SENSORS AND ACTUATORS B-CHEMICAL, 2017, 240 : 100 - 106
  • [5] A protein-based electrochemical biosensor for detection of tau protein, a neurodegenerative disease biomarker
    Esteves-Villanueva, Jose O.
    Trzeciakiewicz, Hanna
    Martic, Sanela
    ANALYST, 2014, 139 (11) : 2823 - 2831
  • [6] Binding, Release, and Detection of Ligands by Ultrabithorax Protein-Based Materials
    Booth, Rebecca
    Churion, Kelly
    Bystroff, Chris
    Bondos, Sarah
    FASEB JOURNAL, 2020, 34
  • [7] Protein-based fibers containing gold nanoparticles as a platform for protease detection
    Abuladel, Nouf
    Bible, Michael
    Bonan, Nicole
    Escobar, Melvin Colorado
    Diaz-Jimenez, Roberta
    Friedel, Benjamin
    Jaffe, Maxi
    Long, Andrew
    Lymperopoulos, Loukas
    Marshall, Sydney
    Nunziata, Jamie
    Rodriguez, Rebeca
    Skorski, Matthew
    Tucker, Asya
    Fox, Douglas
    Hartings, Matthew
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2016, 252
  • [8] A Bacteriophage Protein-Based Impedimetric Electrochemical Biosensor for the Detection of Campylobacter jejuni
    Suganthan, Baviththira
    Rogers, Ashley M.
    Crippen, Clay S.
    Asadi, Hamid
    Zolti, Or
    Szymanski, Christine M.
    Ramasamy, Ramaraja P.
    BIOSENSORS-BASEL, 2024, 14 (08):
  • [9] Electrochemical Aptasensing Platform for the Detection of Retinol Binding Protein-4
    Malecka-Baturo, Kamila
    Zoltowska, Paulina
    Jackowska, Agnieszka
    Kurzatkowska-Adaszynska, Katarzyna
    Grabowska, Iwona
    BIOSENSORS-BASEL, 2024, 14 (02):
  • [10] Reagentless protein-based electrochemical biosensors
    Silva, Saimon M. M.
    Li, Miaosi
    Mendes, Alexandre Xavier
    Moulton, Simon E. E.
    ANALYST, 2023, 148 (09) : 1930 - 1938
12345