A novel DNA binding protein-based platform for electrochemical detection of miRNA

被引：7

作者：

Umer, Muhammad ^{[1
]}

Aziz, Nahian B. ^{[2
]}

Mahmudunnabi, Rabbee G. ^{[3
,4
]}

Shim, Yoon-Bo ^{[3
,4
]}

Salomon, Carlos ^{[2
]}

Shiddiky, Muhammad J. A. ^{[1
,5
]}

机构：

[1] Griffith Univ, Queensland Micro & Nanotechnol Ctr QMNC, Nathan, Qld 4111, Australia

[2] Univ Queensland, Royal Brisbane & Womens Hosp, Ctr Clin Res, Exosome Biol Lab,Ctr Clin Diagnost, Brisbane, Qld, Australia

[3] Pusan Natl Univ, Dept Chem, Busan 46241, South Korea

[4] Pusan Natl Univ, Inst BioPhysio Sensor Technol, Busan 46241, South Korea

[5] Griffith Univ, Sch Environm & Sci, Nathan, Qld 4111, Australia

来源：

ANALYST | 2021年 / 146卷 / 18期

基金：

新加坡国家研究基金会; 英国医学研究理事会; 澳大利亚研究理事会;

关键词：

AMPLIFICATION; REPLICATION; POLYMERASE;

D O I：

10.1039/d1an00935d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a novel amplification-free sandwich type platform assay for electrochemical detection of miRNA. The assay is based on T4 DNA polymerase mediated synthesis of the p53 binding DNA sequence at the 3 ' end of target miRNA. The resulting miRNA-DNA chimera is detected via an electrochemical sandwich hybridization assay where HRP-labelled p53 binds to its recognition sequence and an amperometric signal is generated by hydroquinone-mediated enzymatic reduction of H2O2. The limit of detection of our assay was estimated to be 22 fM with a linear dynamic range of 100 fM-1 nM. This new platform method of detecting miRNA shows superior performance to conventional electrochemical miRNA biosensors and has the potential for amplification-free analysis of miRNA with high specificity and sensitivity.

引用

页码：5496 / +

页数：7

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