A novel DNA binding protein-based platform for electrochemical detection of miRNA

被引：7
|
作者：
Umer, Muhammad [1 ]
Aziz, Nahian B. [2 ]
Mahmudunnabi, Rabbee G. [3 ,4 ]
Shim, Yoon-Bo [3 ,4 ]
Salomon, Carlos [2 ]
Shiddiky, Muhammad J. A. [1 ,5 ]
机构：
[1] Griffith Univ, Queensland Micro & Nanotechnol Ctr QMNC, Nathan, Qld 4111, Australia
[2] Univ Queensland, Royal Brisbane & Womens Hosp, Ctr Clin Res, Exosome Biol Lab,Ctr Clin Diagnost, Brisbane, Qld, Australia
[3] Pusan Natl Univ, Dept Chem, Busan 46241, South Korea
[4] Pusan Natl Univ, Inst BioPhysio Sensor Technol, Busan 46241, South Korea
[5] Griffith Univ, Sch Environm & Sci, Nathan, Qld 4111, Australia
来源：
ANALYST | 2021年 / 146卷 / 18期
基金：
新加坡国家研究基金会; 英国医学研究理事会; 澳大利亚研究理事会;
关键词：
AMPLIFICATION; REPLICATION; POLYMERASE;
D O I：
10.1039/d1an00935d
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We present a novel amplification-free sandwich type platform assay for electrochemical detection of miRNA. The assay is based on T4 DNA polymerase mediated synthesis of the p53 binding DNA sequence at the 3 ' end of target miRNA. The resulting miRNA-DNA chimera is detected via an electrochemical sandwich hybridization assay where HRP-labelled p53 binds to its recognition sequence and an amperometric signal is generated by hydroquinone-mediated enzymatic reduction of H2O2. The limit of detection of our assay was estimated to be 22 fM with a linear dynamic range of 100 fM-1 nM. This new platform method of detecting miRNA shows superior performance to conventional electrochemical miRNA biosensors and has the potential for amplification-free analysis of miRNA with high specificity and sensitivity.
引用
收藏
页码：5496 / +
页数：7
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