PROTEIN-BASED ELECTROCHEMICAL BIOSENSOR FOR DETECTION OF SILVER(I) IONS

被引:24
|
作者
Krizkova, Sona [1 ]
Huska, Dalibor [1 ]
Beklova, Miroslava [2 ]
Hubalek, Jaromir [3 ]
Adam, Vojtech [1 ,4 ]
Trnkova, Libuse [5 ]
Kizek, Rene [1 ]
机构
[1] Mendel Univ Brno, Dept Chem & Biochem, CZ-61300 Brno, Czech Republic
[2] Univ Vet & Pharmaceut Sci, Fac Vet Hyg & Ecol, Dept Vet Ecol & Environm Protect, CZ-61242 Brno, Czech Republic
[3] Brno Univ Technol, Fac Elect Engn & Commun, Dept Microelect, CZ-60200 Brno, Czech Republic
[4] Mendel Univ Brno, Fac Agron, Dept Anim Nutr & Forage Prod, CZ-61300 Brno, Czech Republic
[5] Masaryk Univ, Fac Sci, Dept Chem, CZ-61137 Brno, Czech Republic
关键词
Silver; Biosensors; Voltammetry; Interaction; ATOMIC-ABSORPTION-SPECTROMETRY; TROUT ONCORHYNCHUS-MYKISS; SOLID-PHASE EXTRACTION; RAINBOW-TROUT; METAL-IONS; FATHEAD MINNOWS; AQUEOUS SAMPLES; SODIUM-CHLORIDE; WATER-QUALITY; HEAVY-METALS;
D O I
10.1002/etc.77
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Silver(I) ions are extremely toxic to aquatic animals. Hence, monitoring of these ions in the environment is needed. The aim of the present study was to suggest a simple biosensor for silver(I) ions detection. The suggested biosensor is based on the modification of a hanging mercury drop electrode (HMDE) by the heavy metal binding protein metallothionein (MT) for silver(I) ions detection. Metallothionein accumulated for 120 s onto the HMDE surface. After rinsing the electrode, the biosensor (MT modified HMDE) was prepared prior to detection of silver(I) ions. The biosensor was immersed in a solution containing silver(I) ions. These ions were bound to the MT structure. Furthermore, the electrode was rinsed and transferred to a pure supporting electrolyte solution, in which no interference was present. Under these experimental conditions, other signals relating to heavy metals naturally occurring in MT were not detected. This phenomenon confirms the strong affinity of silver(I) ions for MT. The suggested biosensor responded well to higher silver(I) ion concentrations. The relative standard deviation for measurements of concentrations higher than 50 mu M was approximately 2% (n = 8). In the case of concentrations lower than 10 mu M, the relative standard deviation increased to 10% (n = 8). The detection limit (3 signal/noise) for silver(I) ions was estimated as 500 nM. Environ. Toxicol. Chem. 2010; 29: 492-496. (C) 2009 SETAC
引用
收藏
页码:492 / 496
页数:5
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