Determination of oxytetracycline and some impurities in plasma by non-aqueous capillary electrophoresis using solid-phase extraction

被引：12
|
作者：
Hernández, M [1 ]
Borrull, F [1 ]
Calull, M [1 ]
机构：
[1] Univ Rovira & Virgili, Dept Quim Analit & Quim Organ, Tarragona 43005, Spain
来源：
CHROMATOGRAPHIA | 2000年 / 52卷 / 5-6期
关键词：
capillary zone electrophoresis; non-aqueous medium; solid-phase extraction; tetracyclines; plasma samples;
D O I：
10.1007/BF02491017
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The potential of a non-aqueous, capillary electrophoresis (NACE) system for separating oxytetracycline from three of its impurities - tetracycline, 4-epioxytetracycline and 4-epitetracycline - using UV detection has been studied. The running buffer was: 25 mM sodium acetate, 1 mM EDTA. methanesulfonic acid, pH 4,dissolved in MeOH-ACN (50:50, v/v). The method was also used to determine these compounds in pig plasma. A solid-phase extraction (SPE) procedure as a clean-up step has also developed. For this we tested Sep Pak C-18, LiChrolut EN and OASIS cartridges. OASIS cartridges were best. Recoveries were 90 - 100% for all compounds except EOTC which had a recovery of 74%.
引用
收藏
页码：279 / 284
页数：6
相关论文
共 50 条
  • [41] Determination of cimetidine in human plasma by use of coupled-flow injection, solid-phase extraction, and capillary zone electrophoresis
    Luo, JW
    Chen, HW
    He, QH
    CHROMATOGRAPHIA, 2001, 53 (5-6) : 295 - 300
  • [42] A Non-aqueous Capillary Electrophoresis for Determination of Eugenol in Cloves and Dental Preparations
    Błażej Grodner
    Chromatographia, 2022, 85 : 997 - 1005
  • [43] A Non-aqueous Capillary Electrophoresis for Determination of Eugenol in Cloves and Dental Preparations
    Grodner, Blazej
    CHROMATOGRAPHIA, 2022, 85 (10-11) : 997 - 1005
  • [44] Determination of quercetin in tablets by non-aqueous capillary electrophoresis with conductivity detection
    Zheng, YP
    Mo, JY
    Xie, TY
    Huang, JL
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2002, 30 (11) : 1379 - 1382
  • [45] Selectivity enhancement in capillary electrophoresis using non-aqueous media
    Hansen, SH
    Tjornelund, J
    Bjornsdottir, I
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1996, 15 (04) : 175 - 180
  • [46] Quantification of cholesterol in foods using non-aqueous capillary electrophoresis
    Xu, XH
    Li, RK
    Chen, J
    Chen, P
    Ling, XY
    Rao, PF
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 768 (02): : 369 - 373
  • [47] Non-aqueous bonding of leuprorelin to ochratoxin A for peptide-based solid-phase extraction
    Yamato, Naoki
    Kumagai, Noriaki
    Okahira, Momoha
    Kosaka, Satoru
    Kodama, Shuji
    Yamamoto, Ryohei
    Yamamoto, Atsushi
    Takao, Koichiro
    Yamamoto, Masanori
    CHEMICAL COMMUNICATIONS, 2022, 58 (86) : 12106 - 12109
  • [48] Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase Extraction
    Wang, Qian
    Zhao, Rui
    Cui, Yan
    Liu, Cuicui
    Liang, Liya
    Yan, Shijie
    JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, 2018, 2018
  • [49] Determination of pesticides in waters by automatic on-line solid-phase extraction-capillary electrophoresis
    Hinsmann, P
    Arce, L
    Ríos, A
    Valcárcel, M
    JOURNAL OF CHROMATOGRAPHY A, 2000, 866 (01) : 137 - 146
  • [50] Determination of sulfonamide antibiotics by magnetic porous carbon solid-phase extraction coupled with capillary electrophoresis
    Zhang, Maosen
    Yang, Hanyu
    Yang, Kaijing
    Yang, Qiang
    Liu, Wei
    Yang, Xiupei
    JOURNAL OF CHROMATOGRAPHY A, 2024, 1725
12345