Determination of oxytetracycline and some impurities in plasma by non-aqueous capillary electrophoresis using solid-phase extraction

被引：12
|
作者：
Hernández, M [1 ]
Borrull, F [1 ]
Calull, M [1 ]
机构：
[1] Univ Rovira & Virgili, Dept Quim Analit & Quim Organ, Tarragona 43005, Spain
来源：
CHROMATOGRAPHIA | 2000年 / 52卷 / 5-6期
关键词：
capillary zone electrophoresis; non-aqueous medium; solid-phase extraction; tetracyclines; plasma samples;
D O I：
10.1007/BF02491017
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The potential of a non-aqueous, capillary electrophoresis (NACE) system for separating oxytetracycline from three of its impurities - tetracycline, 4-epioxytetracycline and 4-epitetracycline - using UV detection has been studied. The running buffer was: 25 mM sodium acetate, 1 mM EDTA. methanesulfonic acid, pH 4,dissolved in MeOH-ACN (50:50, v/v). The method was also used to determine these compounds in pig plasma. A solid-phase extraction (SPE) procedure as a clean-up step has also developed. For this we tested Sep Pak C-18, LiChrolut EN and OASIS cartridges. OASIS cartridges were best. Recoveries were 90 - 100% for all compounds except EOTC which had a recovery of 74%.
引用
收藏
页码：279 / 284
页数：6
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