Determination of oxytetracycline and some impurities in plasma by non-aqueous capillary electrophoresis using solid-phase extraction

被引：12
|
作者：
Hernández, M [1 ]
Borrull, F [1 ]
Calull, M [1 ]
机构：
[1] Univ Rovira & Virgili, Dept Quim Analit & Quim Organ, Tarragona 43005, Spain
来源：
CHROMATOGRAPHIA | 2000年 / 52卷 / 5-6期
关键词：
capillary zone electrophoresis; non-aqueous medium; solid-phase extraction; tetracyclines; plasma samples;
D O I：
10.1007/BF02491017
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The potential of a non-aqueous, capillary electrophoresis (NACE) system for separating oxytetracycline from three of its impurities - tetracycline, 4-epioxytetracycline and 4-epitetracycline - using UV detection has been studied. The running buffer was: 25 mM sodium acetate, 1 mM EDTA. methanesulfonic acid, pH 4,dissolved in MeOH-ACN (50:50, v/v). The method was also used to determine these compounds in pig plasma. A solid-phase extraction (SPE) procedure as a clean-up step has also developed. For this we tested Sep Pak C-18, LiChrolut EN and OASIS cartridges. OASIS cartridges were best. Recoveries were 90 - 100% for all compounds except EOTC which had a recovery of 74%.
引用
收藏
页码：279 / 284
页数：6
相关论文
共 50 条
  • [31] Matrix Solid-Phase Dispersion Extraction and Capillary Electrophoresis Determination of Tetracycline Residues in Milk
    Mu, Guangfen
    Liu, Huitao
    Xu, Lina
    Tian, Lihua
    Luan, Feng
    FOOD ANALYTICAL METHODS, 2012, 5 (01) : 148 - 153
  • [32] Determination of nitroimidazole antibiotics based on dispersive solid-phase extraction combined with capillary electrophoresis
    Yang, Jing
    Chen, Lianfang
    Wang, Qingying
    Mei, Xiaolin
    Yang, Xiupei
    Huo, Feng
    ELECTROPHORESIS, 2023, 44 (7-8) : 634 - 645
  • [33] Simultaneous Determination of Antidepressants by Non-aqueous or Quasi-non-aqueous Capillary Electrophoresis
    Sasajima, Yasuhide
    Lim, Lee Wah
    Takeuchi, Toyohide
    Suenami, Koichi
    Sato, Kiyohito
    Takekoshi, Yuji
    ANALYTICAL SCIENCES, 2010, 26 (06) : 693 - 698
  • [34] Simultaneous Determination of Antidepressants by Non-aqueous or Quasi-non-aqueous Capillary Electrophoresis
    Yasuhide Sasajima
    Lee Wah Lim
    Toyohide Takeuchi
    Koichi Suenami
    Kiyohito Sato
    Yuji Takekoshi
    Analytical Sciences, 2010, 26 : 693 - 698
  • [35] Comparison of aqueous and non-aqueous capillary electrophoresis for quantitative determination of morphine in pharmaceuticals
    Bjornsdottir, I
    Hansen, SH
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1997, 15 (08) : 1083 - 1089
  • [36] Combination of off-line solid-phase extraction and on-column sample stacking for sensitive determination of parabens and p-hydroxybenzoic acid in waters by non-aqueous capillary electrophoresis
    Blanco, Eva
    del Carmen Casais, Maria
    del Carmen Mejuto, Maria
    Cela, Rafael
    ANALYTICA CHIMICA ACTA, 2009, 647 (01) : 104 - 111
  • [37] Solid phase extraction prior to non-aqueous capillary electrophoresis with ultraviolet detection as a valuable strategy for therapeutic drug monitoring of cabozantinib
    Nieta, Lucia Nieto Lopez de la
    Bernardo, Francisco Javier Guzman
    Penalvo, Gregorio Castaneda
    Flores, Juana Rodriguez
    MICROCHEMICAL JOURNAL, 2022, 181
  • [38] Determination of cimetidine in human plasma by use of coupled-flow injection, solid-phase extraction, and capillary zone electrophoresis
    J. -W. Luo
    H. -W. Chen
    Q. -H. He
    Chromatographia, 2001, 53 : 295 - 300
  • [39] Determination of gabapentin in human plasma by capillary electrophoresis-laser induced fluorescence detection with and without solid-phase extraction
    Cao, Liwei
    Liang, Siliu
    Tan, Xiaofang
    Meng, Jianxin
    MICROCHIMICA ACTA, 2012, 178 (3-4) : 285 - 292
  • [40] Determination of gabapentin in human plasma by capillary electrophoresis-laser induced fluorescence detection with and without solid-phase extraction
    Liwei Cao
    Siliu Liang
    Xiaofang Tan
    Jianxin Meng
    Microchimica Acta, 2012, 178 : 285 - 292
12345