Bioorthogonal Turn-On Probes for Imaging Small Molecules inside Living Cells

被引：348
|
作者：
Devaraj, Neal K. [1 ]
Hilderbrand, Scott [1 ]
Upadhyay, Rabi [1 ]
Mazitschek, Ralph [1 ]
Weissleder, Ralph [1 ]
机构：
[1] Massachusetts Gen Hosp, Ctr Syst Biol, Richard B Simches Res Ctr, Boston, MA 02114 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 16期
关键词：
bioorthogonal; reactions; cycloaddition; fluorogenic tags; imaging; tetrazine; COPPER(I)-CATALYZED AZIDE-ALKYNE; CLICK CHEMISTRY; STAUDINGER LIGATION; FLUORESCENT TAXOIDS; CANCER-CELLS; COPPER-FREE; CYCLOADDITION; SYSTEMS; ABSENCE; DYE;
D O I：
10.1002/anie.200906120
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Glowing tags: A series of activatable ("urn-on") tetrazine-conjugated fluorescent probes was developed, which react rapidly in an inverse-electron-demand [4+2] cycloaddition with strained dienophiles such as trans-cyclooctene, thereby strongly increasing the fluorescence intensity (see picture), The novel turn-on probes were applied for intracellular livecell imaging of a microtubuli-binding trans-cyclooctene modified taxol, © 2010 Wiley-VCH Verlag GmbH & Co. KGaA,.
引用
收藏
页码：2869 / 2872
页数：4
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