Ratiometric fluorescence imaging of hypochlorous acid in living cells and zebrafish using a novel phenothiazine-fused HPQ probe

被引:17
|
作者
Cui, Hongxia [1 ]
Hou, Peng [1 ]
Li, Yu [1 ]
Sun, Jingwen [1 ]
Zhang, Haiyan [2 ]
Zheng, Yan [2 ]
Liu, Qi [3 ]
Chen, Song [1 ]
机构
[1] Qiqihar Med Univ, Coll Pharm, Qiqihar 161006, Peoples R China
[2] Qiqihar Med Univ, Sci Res Dept, Qiqihar 161006, Peoples R China
[3] Qiqihar Med Univ, Res Inst Med & Pharm, Qiqihar 161006, Peoples R China
关键词
Ratiometric; Fluorescent probe; Hypochlorite; Phenothiazine; LARGE STOKES SHIFT;
D O I
10.1016/j.jphotochem.2021.113343
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Hypochlorite (ClO- ), as a typical weak acidic ROS, is mainly endogenously produced by myeloperoxidase (MPO) catalyzing hydrogen peroxide (H2O2) and chloride ion (Cl- ) in leukocytes. Imbalance of hypochlorite concentration will not only cause biomolecular oxidation in organism, but also are correlated with a variety of physiological diseases. In this study, a novel ratiometric fluorescence probe PHPQ for detecting ClO- by incorporating the phenothiazine moiety into the HPQ fluorophore was designed and synthesized. The probe PHPQ was shown to be capable of selectively and sensitively monitoring ClO- in a fine ratiometric manner. It also displayed the merits of large blue-shift (98 nm), rapid response (30 s), excellent sensitivity (15 nM) and significant fluorescence ratiometric variations (253-fold). Moreover, PHPQ was successfully utilized for bioimaging exogenous/endogenous ClO- in living cells (MGC-803 and RAW264.7) and in zebrafish.
引用
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页数:7
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