Characterization of CRISPR-Cas systems in Bifidobacterium breve

被引：1
|
作者：
Han, Xiao [1 ,2 ]
Zhou, Xingya [1 ,2 ]
Pei, Zhangming [1 ,2 ]
Stanton, Catherine [3 ,4 ,5 ]
Ross, R. Paul [3 ,4 ]
Zhao, Jianxin [1 ,2 ,6 ]
Zhang, Hao [1 ,2 ,6 ,7 ,8 ]
Yang, Bo [1 ,2 ,3 ]
Chen, Wei [1 ,2 ,6 ]
机构：
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China
[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi, Jiangsu, Peoples R China
[3] Jiangnan Univ, Int Joint Res Lab Pharmabiot & Antibiot Resistanc, Wuxi, Jiangsu, Peoples R China
[4] Univ Coll Cork, APC Microbiome Ireland, Cork, Ireland
[5] Teagasc Food Res Ctr, Moorepk, Cork, Ireland
[6] Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi, Jiangsu, Peoples R China
[7] Wuxi Translat Med Res Ctr, Wuxi, Jiangsu, Peoples R China
[8] Jiangsu Translat Med Res Inst, Wuxi Branch, Wuxi, Jiangsu, Peoples R China
来源：
MICROBIAL GENOMICS | 2022年 / 8卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Bifidobacterium breve; CRISPR-Cas system; genotyping; prophage; SPACER ACQUISITION; DOUBLE-BLIND; SUPPLEMENTATION; CLASSIFICATION; CHILDREN; BACTERIA; INFANTS; VERSION; GENOME; GUT;
D O I：
10.1099/mgen.0.000812
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) system is an important adaptive immune system for bacteria to resist foreign DNA infection, which has been widely used in genotyping and gene editing. To provide a theoretical basis for the application of the CRISPR-Cas system in Bifidobacterium breve, the occurrence and diversity of CRISPR-Cas systems were analysed in 150 B. breve strains. Specifically, 47% (71/150) of B. breve genomes possessed the CRISPR-Cas system, and type I-C CRISPR-Cas system was the most widely distributed among those strains. The spacer sequences present in B. breve can be used as a genotyping marker. Additionally, the phage assembly-related proteins were important targets of the type I-C CRISPR-Cas system in B. breve, and the protospacer adjacent motif sequences were further characterized in B. breve type I-C system as 5'-TTC-3'. All these results might provide a molecular basis for the development of endogenous genome editing tools in B. breve.
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收藏
页数：12
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