A ratiometric fluorescent probe for monitoring pH fluctuations during autophagy in living cells

被引：38
|
作者：
Wang, Shan [1 ,2 ]
Wang, Bingya [2 ]
Zhu, Lei [1 ]
Hou, Ji-Ting [2 ]
Yu, Kang-Kang [3 ]
机构：
[1] Hubei Engn Univ, Sch Chem & Mat Sci, Xiaogan 432000, Peoples R China
[2] Xinyang Normal Univ, Coll Chem & Chem Engn, Xinyang 464000, Peoples R China
[3] Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ, Chengdu 610064, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2021年 / 57卷 / 12期
基金：
中国国家自然科学基金;
关键词：
Cell death - Fluorescence;
D O I：
10.1039/d0cc07788g
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We present a ratiometric fluorescent probe for monitoring pH featuring superb photostability and chemostability. The down-regulation of the intracellular pH during autophagy in living cells induced by various stimuli, including several drugs and starvation, was quantified, which could provide instructive value to construct autophagy models to study the related pathological processes.
引用
收藏
页码：1510 / 1513
页数：4
相关论文
共 50 条
  • [21] A ratiometric fluorescent probe for quantification of alkaline phosphatase in living cells
    Lu, Zhenxing
    Wu, Jiasheng
    Liu, Weimin
    Zhang, Guangyou
    Wang, Pengfei
    [J]. RSC ADVANCES, 2016, 6 (38) : 32046 - 32051
  • [22] A ratiometric fluorescent probe for imaging hydroxyl radicals in living cells
    Soh, N
    Makihara, K
    Sakoda, E
    Imato, T
    [J]. CHEMICAL COMMUNICATIONS, 2004, (05) : 496 - 497
  • [23] A ratiometric fluorescent probe for cysteine and its application in living cells
    Dai, Xi
    Zhang, Tao
    Liu, Yi-Zhi
    Yan, Tao
    Li, Yun
    Miao, Jun-Ying
    Zhao, Bao-Xiang
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2015, 207 : 872 - 877
  • [24] A dual-channel fluorescent probe for monitoring pH changes in lysosomes during autophagy
    Huo, Yonghui
    Liang, Xing
    Yan, Jun
    Huang, Ling
    Lin, Weiying
    [J]. NEW JOURNAL OF CHEMISTRY, 2021, 45 (39) : 18538 - 18543
  • [25] An ICT-based fluorescent probe for ratiometric monitoring the fluctuations of peroxynitrite in mitochondria
    Cui, Jie
    Zang, Shunping
    Nie, Hailiang
    Shen, Tiaojiao
    Su, Sa
    Jing, Jing
    Zhang, Xiaoling
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2021, 328
  • [26] A Fluorescence Ratiometric Probe for Imaging of Acidic pH in Living Cells
    Long, Lingliang
    Wu, Yanjun
    Wang, Lin
    [J]. ENVIRONMENTAL ENGINEERING, PTS 1-4, 2014, 864-867 : 83 - 87
  • [27] β-Naphthothiazolium-based ratiometric fluorescent probe with ideal pKa for pH imaging in mitochondria of living cells
    Lin, Bo
    Wei, Yanan
    Hao, Yanan
    Shuang, E.
    Shu, Yang
    Wang, Jianhua
    [J]. TALANTA, 2021, 232
  • [28] Real-time visualization of autophagy by monitoring the fluctuation of lysosomal pH with a ratiometric two-photon fluorescent probe
    Ning, Peng
    Hou, Liling
    Feng, Yan
    Xu, Guoyong
    Bai, Yuyuan
    Yu, Haizhu
    Meng, Xiangming
    [J]. CHEMICAL COMMUNICATIONS, 2019, 55 (12) : 1782 - 1785
  • [29] Mitochondria -targeted reversible ratiometric fluorescent probe for monitoring S02/HCHO in living cells
    Zhang, Tao
    Yin, Caixia
    Zhang, Yongbin
    Chao, Jianbin
    Wen, Guangming
    Huo, Fangjun
    [J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2020, 234
  • [30] A convenient fluorescent probe for monitoring lysosomal pH change and imaging mitophagy in living cells
    Xu, Hongkang
    Bu, Yingcui
    Wang, JunJun
    Qu, Min
    Zhang, Jie
    Zhu, Xiaojiao
    Liu, Gang
    Wu, Zhichao
    Chen, Guangmei
    Zhou, Hongping
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2021, 330 (330):
12345