Mitochondria -targeted reversible ratiometric fluorescent probe for monitoring S02/HCHO in living cells

Sulfur dioxide (502) maintains a certain steady state balance in the body, high concentration SO2 will be harmful W human health. Seeking a suitable detection method to monitor sulfur dioxide in real time becomes an urgent requirement owing to the transient nature of sulfur dioxide in organisms. Here, a novel NIR ratiometric fluorescent probe for detection of 502 was developed based on a conjugation of coumarin and indol salt with excellent water solubility. The probe Mito-CI displayed! highly sensitive (69 nM), fast response lime (30 s), large Stokes shift (174 nm) and the NIR fluorescence emission wavelength (655 nm). In the reversibility process of the 503 -probe Mito-CI system induced by HCHO in vitro was also detected. Besides, cell imaging showed that Mito-CI possesses mitochondria -targeted ability. Part cularly, Mito-CI was proved to reversibly detect 502/ HCHO in living cells. 2020 Elsevier B.V. All rights reserved.