A ratiometric fluorescent probe for cysteine and its application in living cells

被引：60
|
作者：
Dai, Xi [1 ]
Zhang, Tao [2 ]
Liu, Yi-Zhi [3 ]
Yan, Tao [1 ]
Li, Yun [1 ]
Miao, Jun-Ying [2 ]
Zhao, Bao-Xiang [1 ]
机构：
[1] Shandong Univ, Sch Chem & Chem Engn, Inst Organ Chem, Jinan 250100, Peoples R China
[2] Shandong Univ, Sch Life Sci, Inst Dev Biol, Jinan 250100, Peoples R China
[3] Shandong Univ, Sch Chem & Chem Engn, Key Lab Colloid & Interface Chem, Jinan 250100, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2015年 / 207卷
基金：
中国国家自然科学基金;
关键词：
Coumarin; Pyrazoline; Ratiometric; Fluorescence; Cysteine; Living cells; ASSISTED ELECTROSTATIC ATTRACTION; SELECTIVE DETECTION; COLORIMETRIC PROBE; BIOLOGICAL-ACTIVITY; HYDROGEN-SULFIDE; RAPID DETECTION; RECENT PROGRESS; FAST-RESPONSE; GLUTATHIONE; HOMOCYSTEINE;
D O I：
10.1016/j.snb.2014.10.082
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A ratiometric fluorescent probe DPCA based on coumarin and pyrazoline was designed and synthesized for sensing cysteine (Cys) in physiological pH. The probe can detect Cys by fluorescent spectrometry within 40 min with a detection limit of 5.08 mu M. The mechanism of DPCA sensing Cys involved conjugate addition/cyclization which was confirmed by HRMS and fluorescence spectra analysis. Moreover, DPCA was able to be applied to complicated conditions with negligible interference from these species and successfully applied in cell imaging. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：872 / 877
页数：6
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