PCR Detection of Anaplasma phagocytophilum and Borrelia burgdorferi in Ixodes persulcatus Ticks in Mongolia

被引：23

作者：

Masuzawa, Toshiyuki ^{[1
]}

Masuda, Shou ^{[1
]}

Fukui, Takashi ^{[1
]}

Okamoto, Yoshihiro ^{[1
]}

Bataa, Jantsandoo ^{[5
]}

Oikawa, Yosaburo ^{[2
]}

Ishiguro, Fubito ^{[3
]}

Takada, Nobuhiro ^{[4
]}

机构：

[1] CIS, Fac Pharm, Lab Microbiol & Immunol, Choshi 2880025, Japan

[2] Kanazawa Med Univ, Lab Med Zool, Kanazawa, Ishikawa 9200293, Japan

[3] Fukui Prefectural Inst Publ Hlth, Fukui 9108551, Japan

[4] Univ Fukui, Fac Med Sci, Sr Fellow Off, Fukui 9101193, Japan

[5] Mongolian Natl Invest Ctr Infect Dis, Ulaanbaatar, Mongolia

来源：

JAPANESE JOURNAL OF INFECTIOUS DISEASES | 2014年 / 67卷 / 01期

基金：

日本学术振兴会;

关键词：

SENSU-LATO; LYME-BORRELIOSIS; WESTERN SIBERIA; AGENT; EHRLICHIA; RODENTS; PREVALENCE; INFECTION; PROVINCE; GARINII;

D O I：

10.7883/yoken.67.47

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A molecular epidemiological survey was conducted to identify the tick-borne disease agents Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Selenge Province, Mongolia. The survey was in response to a suspected A. phagocytophilum infection in a patient. In 2012, a total of 129 questing Ixodes persulcatus adult ticks were sampled by flagging vegetation. A. phagocytophilum and Borrelia spp. were detected by PCR, targeting the 16S rDNA (rrs) and 5S-23S intergenic spacer region, respectively. Infection rates for A. phagocytophilum and B. burgdorferi sensu lato spp. were 6.2% and 55.0%, respectively. Six of the 129 ticks (4.9%) were coinfected with A. phagocytophilum and B. burgdorferi sensu lato. Among Borrelia spp., the highest prevalence rate was that for B. garinii 20047 type (26.3%), followed by B. afzelii (7.8%) and B. garinii NT29 type (7.0%). Furthermore, ticks were detected that were dually infected with B. afzelii and B. garinii 20047 type (7.8%) and B. garinii NT29 and 20047 types (6.2%).

引用

页码：47 / 49

页数：3

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