PCR Detection of Anaplasma phagocytophilum and Borrelia burgdorferi in Ixodes persulcatus Ticks in Mongolia

被引：23
|
作者：
Masuzawa, Toshiyuki [1 ]
Masuda, Shou [1 ]
Fukui, Takashi [1 ]
Okamoto, Yoshihiro [1 ]
Bataa, Jantsandoo [5 ]
Oikawa, Yosaburo [2 ]
Ishiguro, Fubito [3 ]
Takada, Nobuhiro [4 ]
机构：
[1] CIS, Fac Pharm, Lab Microbiol & Immunol, Choshi 2880025, Japan
[2] Kanazawa Med Univ, Lab Med Zool, Kanazawa, Ishikawa 9200293, Japan
[3] Fukui Prefectural Inst Publ Hlth, Fukui 9108551, Japan
[4] Univ Fukui, Fac Med Sci, Sr Fellow Off, Fukui 9101193, Japan
[5] Mongolian Natl Invest Ctr Infect Dis, Ulaanbaatar, Mongolia
来源：
JAPANESE JOURNAL OF INFECTIOUS DISEASES | 2014年 / 67卷 / 01期
基金：
日本学术振兴会;
关键词：
SENSU-LATO; LYME-BORRELIOSIS; WESTERN SIBERIA; AGENT; EHRLICHIA; RODENTS; PREVALENCE; INFECTION; PROVINCE; GARINII;
D O I：
10.7883/yoken.67.47
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A molecular epidemiological survey was conducted to identify the tick-borne disease agents Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Selenge Province, Mongolia. The survey was in response to a suspected A. phagocytophilum infection in a patient. In 2012, a total of 129 questing Ixodes persulcatus adult ticks were sampled by flagging vegetation. A. phagocytophilum and Borrelia spp. were detected by PCR, targeting the 16S rDNA (rrs) and 5S-23S intergenic spacer region, respectively. Infection rates for A. phagocytophilum and B. burgdorferi sensu lato spp. were 6.2% and 55.0%, respectively. Six of the 129 ticks (4.9%) were coinfected with A. phagocytophilum and B. burgdorferi sensu lato. Among Borrelia spp., the highest prevalence rate was that for B. garinii 20047 type (26.3%), followed by B. afzelii (7.8%) and B. garinii NT29 type (7.0%). Furthermore, ticks were detected that were dually infected with B. afzelii and B. garinii 20047 type (7.8%) and B. garinii NT29 and 20047 types (6.2%).
引用
收藏
页码：47 / 49
页数：3
相关论文
共 50 条
  • [31] Simultaneous Occurrence of Borrelia miyamotoi, Borrelia burgdorferi Sensu Lato, Anaplasma phagocytophilum and Rickettsia helvetica in Ixodes ricinus Ticks in Urban Foci in Bratislava, Slovakia
    Vaculova, Tatiana
    Derdakova, Marketa
    Spitalska, Eva
    Vaclav, Radovan
    Chvostac, Michal
    Taragelova, Veronika Rusnakova
    ACTA PARASITOLOGICA, 2019, 64 (01) : 19 - 30
  • [32] Simultaneous Occurrence of Borrelia miyamotoi, Borrelia burgdorferi Sensu Lato, Anaplasma phagocytophilum and Rickettsia helvetica in Ixodes ricinus Ticks in Urban Foci in Bratislava, Slovakia
    Tatiana Vaculová
    Markéta Derdáková
    Eva Špitalská
    Radovan Václav
    Michal Chvostáč
    Veronika Rusňáková Tarageľová
    Acta Parasitologica, 2019, 64 : 19 - 30
  • [33] CHARACTERIZATION OF BORRELIA-BURGDORFERI ISOLATED FROM IXODES-PERSULCATUS AND IXODES-OVATUS TICKS IN JAPAN
    NAKAO, M
    MIYAMOTO, K
    UCHIKAWA, K
    FUJITA, H
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1992, 47 (04): : 505 - 511
  • [34] Detection of Borrelia burgdorferi, Ehrlichia chaffeensis, and Anaplasma phagocytophilum in ticks (Acari: Ixodidae) from a coastal region of California
    Holden, K
    Boothby, JT
    Anand, S
    Massung, RF
    JOURNAL OF MEDICAL ENTOMOLOGY, 2003, 40 (04) : 534 - 539
  • [35] Borrelia burgdorferi sensu lato in the female cement plug of Ixodes persulcatus ticks (Acari, Ixodidae)
    Alekseev, AN
    Arumova, EA
    Vasilieva, IS
    EXPERIMENTAL & APPLIED ACAROLOGY, 1995, 19 (09): : 519 - 522
  • [36] Prevalence of Borrelia burgdorferi and Anaplasma phagocytophilum in Ixodes ricinus collected from dogs in eastern Poland
    Panczuk, Anna
    Tokarska-Rodak, Malgorzata
    Andrzejuk, Patrycja
    JOURNAL OF VETERINARY RESEARCH, 2024, 68 (01) : 109 - 114
  • [37] Characterization of Anaplasma phagocytophila and Borrelia burgdorferi genotypes in Ixodes scapularis ticks from Pennsylvania
    Courtney, JW
    Dryden, RL
    Wyleto, P
    Schneider, BS
    Massung, RF
    RICKETTSIOLOGY: PRESENT AND FUTURE DIRECTIONS, 2003, 990 : 131 - 133
  • [38] Detection of Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, Borrelia miyamotoi, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
    Tokarz, Rafal
    Tagliafierro, Teresa
    Cucura, D. Moses
    Rochlin, Ilia
    Sameroff, Stephen
    Lipkin, W. Ian
    MSPHERE, 2017, 2 (02):
  • [39] Detection of Anaplasma phagocytophilum, Babesia odocoilei, Babesia sp., Borrelia burgdorferi Sensu Lato, and Hepatozoon canis in Ixodes scapularis Ticks Collected in Eastern Canada
    Scott, John D.
    Pesapane, Risa R.
    PATHOGENS, 2021, 10 (10):
  • [40] Spatial patterns of Borrelia burgdorferi, Borrelia miyamotoi and Anaplasma phagocytophilum detected in Ixodes spp. ticks from Canadian companion animals, 2019-2020
    Jacob, Anna E.
    Weese, Jeffrey Scott
    Rosseau, Joyce
    Clow, Katie M.
    ZOONOSES AND PUBLIC HEALTH, 2022, 69 (08) : 944 - 955
12345