PCR Detection of Anaplasma phagocytophilum and Borrelia burgdorferi in Ixodes persulcatus Ticks in Mongolia

被引：23
|
作者：
Masuzawa, Toshiyuki [1 ]
Masuda, Shou [1 ]
Fukui, Takashi [1 ]
Okamoto, Yoshihiro [1 ]
Bataa, Jantsandoo [5 ]
Oikawa, Yosaburo [2 ]
Ishiguro, Fubito [3 ]
Takada, Nobuhiro [4 ]
机构：
[1] CIS, Fac Pharm, Lab Microbiol & Immunol, Choshi 2880025, Japan
[2] Kanazawa Med Univ, Lab Med Zool, Kanazawa, Ishikawa 9200293, Japan
[3] Fukui Prefectural Inst Publ Hlth, Fukui 9108551, Japan
[4] Univ Fukui, Fac Med Sci, Sr Fellow Off, Fukui 9101193, Japan
[5] Mongolian Natl Invest Ctr Infect Dis, Ulaanbaatar, Mongolia
来源：
JAPANESE JOURNAL OF INFECTIOUS DISEASES | 2014年 / 67卷 / 01期
基金：
日本学术振兴会;
关键词：
SENSU-LATO; LYME-BORRELIOSIS; WESTERN SIBERIA; AGENT; EHRLICHIA; RODENTS; PREVALENCE; INFECTION; PROVINCE; GARINII;
D O I：
10.7883/yoken.67.47
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A molecular epidemiological survey was conducted to identify the tick-borne disease agents Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Selenge Province, Mongolia. The survey was in response to a suspected A. phagocytophilum infection in a patient. In 2012, a total of 129 questing Ixodes persulcatus adult ticks were sampled by flagging vegetation. A. phagocytophilum and Borrelia spp. were detected by PCR, targeting the 16S rDNA (rrs) and 5S-23S intergenic spacer region, respectively. Infection rates for A. phagocytophilum and B. burgdorferi sensu lato spp. were 6.2% and 55.0%, respectively. Six of the 129 ticks (4.9%) were coinfected with A. phagocytophilum and B. burgdorferi sensu lato. Among Borrelia spp., the highest prevalence rate was that for B. garinii 20047 type (26.3%), followed by B. afzelii (7.8%) and B. garinii NT29 type (7.0%). Furthermore, ticks were detected that were dually infected with B. afzelii and B. garinii 20047 type (7.8%) and B. garinii NT29 and 20047 types (6.2%).
引用
收藏
页码：47 / 49
页数：3
相关论文
共 50 条
  • [21] Genetic Variability of Anaplasma phagocytophilum in Ixodes persulcatus Ticks and Small Mammals in the Asian Part of Russia
    Rar, Vera A.
    Epikhina, Tamara I.
    Livanova, Natalya N.
    Panov, Victor V.
    Doroschenko, Elena K.
    Pukhovskaya, Natalya M.
    Vysochina, Nelya P.
    Ivanov, Leonid I.
    VECTOR-BORNE AND ZOONOTIC DISEASES, 2011, 11 (08) : 1013 - 1021
  • [22] Anaplasma phagocytophilum, Babesia microti, and Borrelia burgdorferi in Ixodes scapularis, southern coastal Maine
    Holman, MS
    Caporale, DA
    Goldberg, J
    Lacombe, E
    Lubelczyk, C
    Rand, PW
    Smith, RP
    EMERGING INFECTIOUS DISEASES, 2004, 10 (04) : 744 - 746
  • [23] Confirmation of Borrelia burgdorferi sensu stricto and Anaplasma phagocytophilum in Ixodes scapularis, Southwestern Virginia
    Herrin, Brian H.
    Zajac, Anne M.
    Little, Susan E.
    VECTOR-BORNE AND ZOONOTIC DISEASES, 2014, 14 (11) : 821 - 823
  • [24] Detection of Borrelia burgdorferi, Anaplasma phagocytophilum and Babesia microti, with two different multiplex PCR assays
    Hojgaard, Andrias
    Lukacik, Gary
    Piesman, Joseph
    TICKS AND TICK-BORNE DISEASES, 2014, 5 (03) : 349 - 351
  • [25] Prevalence of Anaplasma phagocytophilum, Rickettsia sp and Borrelia burgdorferi sensu lato DNA in questing Ixodes ricinus ticks from France
    Halos, Lenaig
    Vourc'h, Gwenael
    Cotte, Violaine
    Gasqui, Patrick
    Barnouin, Jacques
    Boulous, Henri-Jean
    Vayssier-Taussat, Muriel
    CENTURY OF RICKETTSIOLOGY: EMERGING, REEMERGING RICKETTSIOSES, MOLECULAR DIAGNOSTICS, AND EMERGING VETERINARY RICKETTSIOSES, 2006, 1078 : 316 - 319
  • [26] Prevalence of Borrelia burgdorferi, bartonella spp., Babesia microti, and Anaplasma phagocytophilum in Ixodes scapularis ticks removed from humans
    Stromdahl, Ellen Y.
    Evans, Sandra R.
    Barry, Ryan K.
    Cai, Cindy
    Vince, Mary A.
    Williamson, Phillip C.
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2006, 75 (05): : 30 - 30
  • [27] Elimination of Borrelia burgdorferi and Anaplasma phagocytophilum in Rodent Reservoirs and Ixodes scapularis Ticks Using a Doxycycline Hyclate-Laden Bait
    Dolan, Marc C.
    Schulze, Terry L.
    Jordan, Robert A.
    Dietrich, Gabrielle
    Schulze, Christopher J.
    Hojgaard, Andrias
    Ullmann, Amy J.
    Sackal, Cherilyn
    Zeidner, Nordin S.
    Piesman, Joseph
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2011, 85 (06): : 1114 - 1120
  • [28] COINCIDENCE OF THREE PATHOGENS (BORRELIA BURGDORFERI SENSU LATO, ANAPLASMA PHAGOCYTOPHILUM AND BABESIA MICROTI) IN IXODES RICINUS TICKS IN THE LUBLIN MACROREGION
    Wojcik-Fatla, Angelina
    Szymanska, Jolanta
    Wdowiak, Leszek
    Buczek, Alicja
    Dutkiewicz, Jacek
    ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE, 2009, 16 (01) : 151 - 158
  • [29] Prevalence of Borrelia burgdorferi, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected from the Lublin region (eastern Poland)
    Cisak, E.
    Chmielewska-Badora, J.
    Wojcik-Fatla, A.
    Zwolinski, J.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2007, 29 : S421 - S421
  • [30] Detection of Borrelia burgdorferi and Anaplasma phagocytophilum in the black-legged tick, Ixodes scapularis, within southwestern Pennsylvania
    Brown, Scott M.
    Lehman, Preston M.
    Kern, Ryan A.
    Henning, Jill D.
    JOURNAL OF VECTOR ECOLOGY, 2015, 40 (01) : 180 - 183
12345