Quantitation of five organophosphorus nerve agent metabolites in serum using hydrophilic interaction liquid chromatography and tandem mass spectrometry

被引:42
|
作者
Hamelin, Elizabeth I. [1 ]
Schulze, Nicholas D. [2 ]
Shaner, Rebecca L. [1 ]
Coleman, Rebecca M. [2 ]
Lawrence, Richard J. [3 ]
Crow, Brian S. [1 ]
Jakubowski, E. M. [3 ]
Johnson, Rudolph C. [1 ]
机构
[1] Ctr Dis Control & Prevent, Div Sci Lab, Natl Ctr Environm Hlth, Atlanta, GA 30341 USA
[2] ORISE, Oak Ridge, TN 37831 USA
[3] US Army Edgewood Chem Biol Ctr, R&T Directorate, Aberdeen, MD 21010 USA
关键词
Organophosphorus nerve agents; Metabolites; Serum; Exposure; ISOPROPYL METHYLPHOSPHONIC ACID; ISOTOPE-DILUTION; HUMAN URINE; DEGRADATION-PRODUCTS; QUANTIFICATION; SAMPLES; SARIN; SOMAN; IONIZATION; EXTRACTION;
D O I
10.1007/s00216-014-7702-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Although nerve agent use is prohibited, concerns remain for human exposure to nerve agents during decommissioning, research, and warfare. Exposure can be detected through the analysis of hydrolysis products in urine as well as blood. An analytical method to detect exposure to five nerve agents, including VX, VR (Russian VX), GB (sarin), GD (soman), and GF (cyclosarin), through the analysis of the hydrolysis products, which are the primary metabolites, in serum has been developed and characterized. This method uses solid-phase extraction coupled with high-performance liquid chromatography for separation and isotopic dilution tandem mass spectrometry for detection. An uncommon buffer of ammonium fluoride was used to enhance ionization and improve sensitivity when coupled with hydrophilic interaction liquid chromatography resulting in detection limits from 0.3 to 0.5 ng/mL. The assessment of two quality control samples demonstrated high accuracy (101-105 %) and high precision (5-8 %) for the detection of these five nerve agent hydrolysis products in serum.
引用
收藏
页码:5195 / 5202
页数:8
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