Mechanism of action of DNA-hydrolyzing antibodies to DNA from blood of patients with systemic lupus erythematosus

被引：4
|
作者：
Nevzorova, T. A. [1 ]
Vinter, V. G. [1 ]
Konovalova, O. A. [1 ]
Salakhov, M. Kh. [1 ]
机构：
[1] Kazan VI Lenin State Univ, Kazan 420008, Russia
来源：
BIOCHEMISTRY-MOSCOW | 2006年 / 71卷 / 11期
关键词：
polyclonal catalytic antibodies; abzymes; systemic lupus erythematosus; IgG; hydrolysis of DNA; atomic-force microscopy;
D O I：
10.1134/S0006297906110095
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Four fractions of IgG antibodies to native DNA (nDNA) were obtained from blood of patients with systemic lupus erythematosus. These antibodies displayed a thermostable DNA-hydrolyzing activity and were different in affinity for DNA-cellulose and sorption on DEAE-cellulose. DNA-hydrolyzing antibodies to nDNA are metal-dependent endonucleases, cause mainly single-strand breaks in DNA, and are active over a wide range of pH. By atomic-force microscopy, three-dimensional images of DNA complexes with DNA-hydrolyzing antibodies to nDNA were obtained with nanometer resolution, and a nonprocessive action mechanism was shown for the DNase activity of antibodies to nDNA.
引用
收藏
页码：1238 / 1246
页数：9
相关论文
共 50 条
  • [41] ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS-ERYTHEMATOSUS
    CLOTET, B
    RUIBAL, A
    PIGRAU, C
    MURCIA, C
    PAHISSA, A
    LIENCE, E
    MEDICINA CLINICA, 1982, 78 (04): : 128 - 131
  • [42] ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS-ERYTHEMATOSUS
    PISETSKY, DS
    RHEUMATIC DISEASE CLINICS OF NORTH AMERICA, 1992, 18 (02) : 437 - 454
  • [43] ASSAY AND USE OF ANTIBODIES TO DNA IN SYSTEMIC LUPUS-ERYTHEMATOSUS
    HARLEY, EH
    LUTTON, D
    HALL, E
    LOSMAN, ML
    MEYERS, OL
    SOUTH AFRICAN MEDICAL JOURNAL, 1976, 50 (26): : 1010 - 1010
  • [44] ISOLATION AND CHARACTERIZATION OF DNA-SPECIFIC CATALYTIC ANTIBODIES FROM PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
    GOLOLOBOV, GV
    BOGOMOLOVA, AE
    YADAV, RP
    ERMOLAEVA, MV
    BELOSTOTSKAYA, KM
    PROKAEVA, TB
    SHUSTER, AM
    GABIBOV, AG
    BIOCHEMISTRY-MOSCOW, 1993, 58 (02) : 183 - 187
  • [45] ANTI-DNA ANTIBODIES OF IGA CLASS IN PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
    GRIPENBERG, M
    HELVE, T
    RHEUMATOLOGY INTERNATIONAL, 1986, 6 (02) : 53 - 55
  • [46] ANTI-DNA ANTIBODIES AND RENAL LESIONS OF PATIENTS WITH SYSTEMIC-LUPUS-ERYTHEMATOSUS
    KOFFLER, D
    AGNELLO, V
    CARR, RI
    KUNKEL, HG
    TRANSPLANTATION PROCEEDINGS, 1969, 1 (04) : 933 - &
  • [47] ANTIBODIES CROSS-REACTIVE WITH DNA AND CARDIOLIPIN IN PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
    KOIKE, T
    TOMIOKA, H
    KUMAGAI, A
    CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 1982, 50 (02): : 298 - 302
  • [48] ENTRAPMENT OF ANTI-DNA ANTIBODIES IN THE KIDNEY OF PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
    YAMADA, A
    MIYAKAWA, Y
    KOSAKA, K
    KIDNEY INTERNATIONAL, 1982, 22 (06) : 671 - 676
  • [49] RESPONSE OF CULTURED LYMPHOCYTES FROM PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS TO DNA
    PATRUCCO, A
    ROTHFIELD, NF
    HIRSCHHORN, K
    ARTHRITIS AND RHEUMATISM, 1967, 10 (01): : 32 - +
  • [50] DETECTION OF COMPLEXES BETWEEN DNA AND ANTI-DNA ANTIBODIES IN SERUM OF PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
    HARLEY, EH
    LUTTON, D
    MEYERS, OL
    SOUTH AFRICAN MEDICAL JOURNAL, 1977, 51 (25): : 935 - 935
12345