Mechanism of action of DNA-hydrolyzing antibodies to DNA from blood of patients with systemic lupus erythematosus

被引：4

作者：

Nevzorova, T. A. ^{[1
]}

Vinter, V. G. ^{[1
]}

Konovalova, O. A. ^{[1
]}

Salakhov, M. Kh. ^{[1
]}

机构：

[1] Kazan VI Lenin State Univ, Kazan 420008, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2006年 / 71卷 / 11期

关键词：

polyclonal catalytic antibodies; abzymes; systemic lupus erythematosus; IgG; hydrolysis of DNA; atomic-force microscopy;

D O I：

10.1134/S0006297906110095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four fractions of IgG antibodies to native DNA (nDNA) were obtained from blood of patients with systemic lupus erythematosus. These antibodies displayed a thermostable DNA-hydrolyzing activity and were different in affinity for DNA-cellulose and sorption on DEAE-cellulose. DNA-hydrolyzing antibodies to nDNA are metal-dependent endonucleases, cause mainly single-strand breaks in DNA, and are active over a wide range of pH. By atomic-force microscopy, three-dimensional images of DNA complexes with DNA-hydrolyzing antibodies to nDNA were obtained with nanometer resolution, and a nonprocessive action mechanism was shown for the DNase activity of antibodies to nDNA.

引用

页码：1238 / 1246

页数：9

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