Mechanism of action of DNA-hydrolyzing antibodies to DNA from blood of patients with systemic lupus erythematosus

被引：4
|
作者：
Nevzorova, T. A. [1 ]
Vinter, V. G. [1 ]
Konovalova, O. A. [1 ]
Salakhov, M. Kh. [1 ]
机构：
[1] Kazan VI Lenin State Univ, Kazan 420008, Russia
来源：
BIOCHEMISTRY-MOSCOW | 2006年 / 71卷 / 11期
关键词：
polyclonal catalytic antibodies; abzymes; systemic lupus erythematosus; IgG; hydrolysis of DNA; atomic-force microscopy;
D O I：
10.1134/S0006297906110095
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Four fractions of IgG antibodies to native DNA (nDNA) were obtained from blood of patients with systemic lupus erythematosus. These antibodies displayed a thermostable DNA-hydrolyzing activity and were different in affinity for DNA-cellulose and sorption on DEAE-cellulose. DNA-hydrolyzing antibodies to nDNA are metal-dependent endonucleases, cause mainly single-strand breaks in DNA, and are active over a wide range of pH. By atomic-force microscopy, three-dimensional images of DNA complexes with DNA-hydrolyzing antibodies to nDNA were obtained with nanometer resolution, and a nonprocessive action mechanism was shown for the DNase activity of antibodies to nDNA.
引用
收藏
页码：1238 / 1246
页数：9
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