Ligand-responsive upregulation of 3' CITE-mediated translation in a wheat germ cell-free expression system

被引：15
|
作者：
Ogawa, Atsushi [1 ]
Murashige, Yuta [1 ]
Tabuchi, Junichiro [1 ]
Omatsu, Taiki [1 ]
机构：
[1] Ehime Univ, Proteosci Ctr, 3 Bunkyo Cho, Matsuyama, Ehime 7908577, Japan
来源：
MOLECULAR BIOSYSTEMS | 2017年 / 13卷 / 02期
关键词：
PROTEIN-SYNTHESIS SYSTEM; UNTRANSLATED REGIONS; REPORTER PROTEIN; PLANT-VIRUSES; TRANSFER-RNA; RIBOSWITCHES; EXTRACT; BIOLOGY; RESOLUTION; ENHANCERS;
D O I：
10.1039/c6mb00748a
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 30 cap-independent translation element (30 CITE) with no major hybridization switches in a plant expression system (wheat germ extract).
引用
收藏
页码：314 / 319
页数：6
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