Ligand-responsive upregulation of 3' CITE-mediated translation in a wheat germ cell-free expression system

被引：15
|
作者：
Ogawa, Atsushi [1 ]
Murashige, Yuta [1 ]
Tabuchi, Junichiro [1 ]
Omatsu, Taiki [1 ]
机构：
[1] Ehime Univ, Proteosci Ctr, 3 Bunkyo Cho, Matsuyama, Ehime 7908577, Japan
来源：
MOLECULAR BIOSYSTEMS | 2017年 / 13卷 / 02期
关键词：
PROTEIN-SYNTHESIS SYSTEM; UNTRANSLATED REGIONS; REPORTER PROTEIN; PLANT-VIRUSES; TRANSFER-RNA; RIBOSWITCHES; EXTRACT; BIOLOGY; RESOLUTION; ENHANCERS;
D O I：
10.1039/c6mb00748a
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 30 cap-independent translation element (30 CITE) with no major hybridization switches in a plant expression system (wheat germ extract).
引用
收藏
页码：314 / 319
页数：6
相关论文
共 50 条
  • [21] Ribosomal RNA supplementation highly reinforced cell-free translation activity of wheat germ
    Shen, XC
    Yao, SL
    Fukano, H
    Kitayama, A
    Nagamune, T
    Suzuki, E
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2000, 89 (01) : 68 - 72
  • [22] ANALYSIS OF PLANT POLY(A)-MESSENGER RNA TRANSLATION PRODUCTS SYNTHESIZED IN A CELL-FREE WHEAT-GERM SYSTEM
    BRALCZYK, J
    WASILEWSKA, LD
    ACTA PHYSIOLOGIAE PLANTARUM, 1990, 12 (02) : 159 - 166
  • [23] Enhancement of the catalytic activity of the wheat germ, cell-free, protein synthesis system using a fortified translation extract
    Park, Young Seoub
    Chu, Hun Su
    Choi, Cha Yong
    Jung, Gyoo Yeol
    NEW DEVELOPMENT AND APPLICATION IN CHEMICAL REACTION ENGINEERING, 4TH ASIA-PACIFIC CHEMICAL REACTION ENGINEERING SYMPOSIUM (APCRE 05), 2006, 159 : 169 - 172
  • [24] PREPARATION OF MOUSE KIDNEY POLY-A RNA AND ITS TRANSLATION IN A WHEAT-GERM CELL-FREE SYSTEM
    ZELNICK, CJ
    MEREDITH, SA
    GANSCHOW, RE
    CLINICAL RESEARCH, 1977, 25 (04): : A635 - A635
  • [25] Stimulation of the Translation of Reporter mRNA in the Presence of Another mRNA in a Cell-Free System Based on Wheat Germ Extract
    E. A. Sogorin
    S. Ch. Agalarov
    Molecular Biology, 2018, 52 : 15 - 18
  • [26] Stimulation of the Translation of Reporter mRNA in the Presence of Another mRNA in a Cell-Free System Based on Wheat Germ Extract
    Sogorin, E. A.
    Agalarov, S. Ch.
    MOLECULAR BIOLOGY, 2018, 52 (01) : 15 - 18
  • [27] Application of the wheat-germ cell-free translation system to produce high temperature requirement A3 (HtrA3) proteases
    Singh, Harmeet
    Makino, Shin-ichi
    Endo, Yaeta
    Li, Ying
    Stephens, Andrew N.
    Nie, Guiying
    BIOTECHNIQUES, 2012, 52 (01) : 23 - 28
  • [28] TRANSLATION OF EXOGENEOUS MESSENGER-RNA IN A WHEAT EMBRYO CELL-FREE SYSTEM - EFFECT OF COMPOSITION OF CELL-FREE SYSTEM
    BOROVA, J
    FUCHS, O
    PONKA, P
    NEUWIRT, J
    COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 1978, 43 (03) : 900 - 906
  • [29] Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline
    Guild, Katherine
    Zhang, Yang
    Stacy, Robin
    Mundt, Elizabeth
    Benbow, Sarah
    Green, Amanda
    Myler, Peter J.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 1027 - 1031
  • [30] Application of wheat germ cell-free protein expression system for novel malaria vaccine candidate discovery
    Arumugam, Thangavelu U.
    Ito, Daisuke
    Takashima, Eizo
    Tachibana, Mayumi
    Ishino, Tomoko
    Torii, Motomi
    Tsuboi, Takafumi
    EXPERT REVIEW OF VACCINES, 2014, 13 (01) : 75 - 85
12345