Ligand-responsive upregulation of 3' CITE-mediated translation in a wheat germ cell-free expression system

被引：15

作者：

Ogawa, Atsushi ^{[1
]}

Murashige, Yuta ^{[1
]}

Tabuchi, Junichiro ^{[1
]}

Omatsu, Taiki ^{[1
]}

机构：

[1] Ehime Univ, Proteosci Ctr, 3 Bunkyo Cho, Matsuyama, Ehime 7908577, Japan

来源：

MOLECULAR BIOSYSTEMS | 2017年 / 13卷 / 02期

关键词：

PROTEIN-SYNTHESIS SYSTEM; UNTRANSLATED REGIONS; REPORTER PROTEIN; PLANT-VIRUSES; TRANSFER-RNA; RIBOSWITCHES; EXTRACT; BIOLOGY; RESOLUTION; ENHANCERS;

D O I：

10.1039/c6mb00748a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 30 cap-independent translation element (30 CITE) with no major hybridization switches in a plant expression system (wheat germ extract).

引用

页码：314 / 319

页数：6

共 50 条

[31] TRANSLATION OF TMV-RNA IN A CELL-FREE WHEAT EMBRYO SYSTEM
EFRON, D
MARCUS, A
VIROLOGY, 1973, 53 (02) : 343 - 348
[32] TRANSLATION OF TYPE 1 AND TYPE 2 PROCOLLAGEN MESSENGERS IN A CELL-FREE SYSTEM DERIVED FROM WHEAT-GERM
HARWOOD, R
GRANT, ME
JACKSON, DS
FEBS LETTERS, 1975, 57 (01) : 47 - 50
[33] DIFFERENTIAL TRANSLATION OF RAT-LIVER ALBUMIN MESSENGER-RNA IN A WHEAT-GERM CELL-FREE SYSTEM
SONENSHEIN, GE
BRAWERMAN, G
BIOCHEMISTRY, 1977, 16 (25) : 5445 - 5448
[34] TRANSLATION OF COLLAGEN MESSENGER-RNA IN ASCITES AND WHEAT-GERM CELL-FREE SYSTEMS
BENVENIS.K
WILCZEK, J
STERN, R
FEDERATION PROCEEDINGS, 1974, 33 (05) : 1541 - 1541
[35] TRANSLATION OF MESSENGER-RNA FROM PLASMACYTOMA MOPC 21 CELLS IN A WHEAT-GERM CELL-FREE SYSTEM
GORYUNOVA, LE
SAKHAROVA, NK
BARBAKAR, NI
GRECHKO, VV
MOLECULAR BIOLOGY, 1980, 14 (05) : 929 - 939
[36] CONTROL OF PROTEIN-SYNTHESIS IN A WHEAT-GERM CELL-FREE SYSTEM
SZYBIAK, U
LEGOCKI, AB
ACTA BIOCHIMICA POLONICA, 1983, 30 (3-4) : 255 - &
[37] Formation of circular polyribosomes in wheat germ cell-free protein synthesis system
Madin, K
Sawasaki, T
Kamura, N
Takai, K
Ogasawara, T
Yazaki, K
Takei, T
Miura, KI
Endo, Y
FEBS LETTERS, 2004, 562 (1-3) : 155 - 159
[38] ENDOGENOUS PROTEIN-SYNTHESIS OF CELL-FREE WHEAT-GERM SYSTEM
SENGER, DR
GROSS, PR
DEVELOPMENTAL BIOLOGY, 1976, 53 (01) : 128 - 133
[39] EXPRESSION OF FUNCTIONAL NEUTROPHIL COLLAGENASE IN A CELL-FREE TRANSLATION SYSTEM
DEVARAJAN, P
VANWART, H
BERLINER, N
CLINICAL RESEARCH, 1991, 39 (02): : A269 - A269
[40] Statistical analysis of features associated with protein expression/solubility in an in vivo Escherichia coli expression system and a wheat germ cell-free expression system
Hirose, Shuichi
Kawamura, Yoshifumi
Yokota, Kiyonobu
Kuroita, Toshihiro
Natsume, Tohru
Komiya, Kazuo
Tsutsumi, Takeshi
Suwa, Yorimasa
Isogai, Takao
Goshima, Naoki
Noguchi, Tamotsu
JOURNAL OF BIOCHEMISTRY, 2011, 150 (01): : 73 - 81

← 1 2 3 4 5 →