Ligand-responsive upregulation of 3' CITE-mediated translation in a wheat germ cell-free expression system

被引：15

作者：

Ogawa, Atsushi ^{[1
]}

Murashige, Yuta ^{[1
]}

Tabuchi, Junichiro ^{[1
]}

Omatsu, Taiki ^{[1
]}

机构：

[1] Ehime Univ, Proteosci Ctr, 3 Bunkyo Cho, Matsuyama, Ehime 7908577, Japan

来源：

MOLECULAR BIOSYSTEMS | 2017年 / 13卷 / 02期

关键词：

PROTEIN-SYNTHESIS SYSTEM; UNTRANSLATED REGIONS; REPORTER PROTEIN; PLANT-VIRUSES; TRANSFER-RNA; RIBOSWITCHES; EXTRACT; BIOLOGY; RESOLUTION; ENHANCERS;

D O I：

10.1039/c6mb00748a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 30 cap-independent translation element (30 CITE) with no major hybridization switches in a plant expression system (wheat germ extract).

引用

页码：314 / 319

页数：6

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