Fluorescent oligonucleotides - Versatile tools as probes and primers for DNA and RNA analysis

被引：1
|
作者：
Wojczewski, C
Stolze, K
Engels, JW
机构：
[1] Univ Frankfurt, Inst Organ Chem, D-60439 Frankfurt, Germany
[2] Humboldt Univ, Inst Organ & Bioorgan Chem, D-10115 Berlin, Germany
来源：
SYNLETT | 1999年 / 10期
关键词：
fluorescent oligonucleotides; automated solid-phase synthesis; modified nucleosides; fluorescence spectroscopy; DNA;
D O I：
暂无
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Different strategies for the fluorescence labeling of nucleosides and oligonucleotides and techniques for their detection are discussed. Pre- and post-synthetic routes as well as automated solid phase-based syntheses and enzymatic methods are compared. Tailor-made chemical syntheses are presented that meet the requirements of a broad spectrum of applications for oligonucleotides as probes.
引用
收藏
页码：1667 / 1678
页数：12
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