Fluorescent oligonucleotides - Versatile tools as probes and primers for DNA and RNA analysis

被引：1

作者：

Wojczewski, C

Stolze, K

Engels, JW

机构：

[1] Univ Frankfurt, Inst Organ Chem, D-60439 Frankfurt, Germany

[2] Humboldt Univ, Inst Organ & Bioorgan Chem, D-10115 Berlin, Germany

来源：

SYNLETT | 1999年 / 10期

关键词：

fluorescent oligonucleotides; automated solid-phase synthesis; modified nucleosides; fluorescence spectroscopy; DNA;

D O I：

暂无

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Different strategies for the fluorescence labeling of nucleosides and oligonucleotides and techniques for their detection are discussed. Pre- and post-synthetic routes as well as automated solid phase-based syntheses and enzymatic methods are compared. Tailor-made chemical syntheses are presented that meet the requirements of a broad spectrum of applications for oligonucleotides as probes.

引用

页码：1667 / 1678

页数：12

共 50 条

[21] PLASMID DNA SEQUENCING USING HIGHLY DEGENERATE OLIGONUCLEOTIDES AS PRIMERS
KONRAD, M
NUCLEIC ACIDS RESEARCH, 1990, 18 (17) : 5320 - 5320
[22] 5-amido-(carboxyfluorescein)2'-dU-oligonucleotides: Novel primers for fluorescent detection of PCR amplified DNA
Jadhav, VR
Barawkar, DA
Natu, AA
Ganesh, KN
NUCLEOSIDES & NUCLEOTIDES, 1997, 16 (1-2): : 107 - 114
[23] FLUORESCENT PROBES OF RNA-POLYMERASE
WU, CW
WU, FYH
FEDERATION PROCEEDINGS, 1973, 32 (03) : 645 - &
[24] Use of cadA-Specific Primers and DNA Probes as Tools to Select Cadmium Biosorbents with Potential in Remediation Strategies
Icgen, Bulent
Yilmaz, Fadime
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 2016, 96 (05) : 685 - 693
[25] Use of cadA-Specific Primers and DNA Probes as Tools to Select Cadmium Biosorbents with Potential in Remediation Strategies
Bulent Icgen
Fadime Yilmaz
Bulletin of Environmental Contamination and Toxicology, 2016, 96 : 685 - 693
[26] Fluorescent probes for proteolysis: Tools for drug discovery
Neefjes, J
Dantuma, NP
NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (01) : 58 - 69
[27] Fluorescent probes for proteolysis: Tools for drug discovery
Jacques Neefjes
Nico P. Dantuma
Nature Reviews Drug Discovery, 2004, 3 : 58 - 69
[28] Hydrogen and hydration of DNA and RNA oligonucleotides
Sundaralingam, M
Pan, BC
BIOPHYSICAL CHEMISTRY, 2002, 95 (03) : 273 - 282
[29] Photochemical modifications for DNA/RNA oligonucleotides
Tavakoli, Amirrasoul
Min, Jung-Hyun
RSC ADVANCES, 2022, 12 (11) : 6484 - 6507
[30] Development of Fluorescent Protein Probes Specific for Parallel DNA and RNA G-Quadruplexes
Dung Thanh Dang
Anh Tuan Phan
CHEMBIOCHEM, 2016, 17 (01) : 42 - 45

← 1 2 3 4 5 →