Fluorescent oligonucleotides - Versatile tools as probes and primers for DNA and RNA analysis

被引：1
|
作者：
Wojczewski, C
Stolze, K
Engels, JW
机构：
[1] Univ Frankfurt, Inst Organ Chem, D-60439 Frankfurt, Germany
[2] Humboldt Univ, Inst Organ & Bioorgan Chem, D-10115 Berlin, Germany
来源：
SYNLETT | 1999年 / 10期
关键词：
fluorescent oligonucleotides; automated solid-phase synthesis; modified nucleosides; fluorescence spectroscopy; DNA;
D O I：
暂无
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Different strategies for the fluorescence labeling of nucleosides and oligonucleotides and techniques for their detection are discussed. Pre- and post-synthetic routes as well as automated solid phase-based syntheses and enzymatic methods are compared. Tailor-made chemical syntheses are presented that meet the requirements of a broad spectrum of applications for oligonucleotides as probes.
引用
收藏
页码：1667 / 1678
页数：12
相关论文
共 50 条
  • [31] Phosphoryl guanidine oligonucleotides as primers for RNA-dependent DNA synthesis using murine leukemia virus reverse transcriptase
    Dyudeeva, E. S.
    Pyshnaya, I. A.
    VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII, 2022, 26 (01): : 5 - 13
  • [32] USE OF DNA PROBES AND OLIGONUCLEOTIDES IN PRENATAL-DIAGNOSIS
    MULLER, U
    ARCHIVES OF GYNECOLOGY AND OBSTETRICS, 1993, 254 (1-4) : 1610 - 1618
  • [33] Fluorescent silver nanoclusters as DNA probes
    Obliosca, Judy M.
    Liu, Cong
    Yeh, Hsin-Chih
    NANOSCALE, 2013, 5 (18) : 8443 - 8461
  • [34] Probes in DNA/RNA hybridization
    Longiaru, M
    BIOLOGICALS, 1996, 24 (03) : 187 - 188
  • [35] PADLOCK PROBES - CIRCULARIZING OLIGONUCLEOTIDES FOR LOCALIZED DNA DETECTION
    NILSSON, M
    MALMGREN, H
    SAMIOTAKI, M
    KWIATKOWSKI, M
    CHOWDHARY, BP
    LANDEGREN, U
    SCIENCE, 1994, 265 (5181) : 2085 - 2088
  • [36] Conjugates of oligonucleotides with polyaromatic fluorophores as promising DNA probes
    Balakin, KV
    Korshun, VA
    Mikhalev, II
    Maleev, GV
    Malakhov, AD
    Prokhorenko, IA
    Berlin, YA
    BIOSENSORS & BIOELECTRONICS, 1998, 13 (7-8): : 771 - 778
  • [37] Ligation mediated fluorescent labeling of DNA sequencing primers
    Jang, GY
    Steffens, DL
    NUCLEIC ACIDS RESEARCH, 1997, 25 (04) : 922 - 923
  • [38] Versatile primers for the amplification of the mitochondrial DNA control region in marsupials
    Fumagalli, L
    Pope, LC
    Taberlet, P
    Moritz, C
    MOLECULAR ECOLOGY, 1997, 6 (12) : 1199 - 1201
  • [39] Recurrence time statistics: Versatile tools for genomic DNA sequence analysis
    Cao, Y
    Tung, WW
    Gao, JB
    2004 IEEE COMPUTATIONAL SYSTEMS BIOINFORMATICS CONFERENCE, PROCEEDINGS, 2004, : 40 - 51
  • [40] Fluorescent probes for the analysis of DNA strand scission in base excision repair
    Matsumoto, Naoyuki
    Toga, Tatsuya
    Hayashi, Ryosuke
    Sugasawa, Kaoru
    Katayanagi, Katsuo
    Ide, Hiroshi
    Kuraoka, Isao
    Iwai, Shigenori
    NUCLEIC ACIDS RESEARCH, 2010, 38 (07) : e101
12345