The rapid evaluation of bacterial growth in blood cultures by selected ion flow tube-mass spectrometry (SIFT-MS) and comparison with the BacT/ALERT automated blood culture system

被引:57
|
作者
Scotter, J. M.
Allardyce, R. A.
Langford, Vs.
Hill, A.
Murdoch, D. R.
机构
[1] Syft Technol Ltd, Med Applicat, Christchurch, New Zealand
[2] Univ Otago, Christchurch Sch Med & Hlth Sci, Dunedin, New Zealand
[3] Canterbury Hlth Labs, Microbiol Unit, Christchurch, New Zealand
关键词
blood cultures; selected ion flow tube-mass spectrometry; BacT/ALERT;
D O I
10.1016/j.mimet.2005.09.016
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We compared the performance of the BacT/ALERT automated blood culture system with real-time, quantitative volatile organic compound (VOC) detection by selected ion flow tube-mass spectrometry (SIFT-MS). Blood samples from healthy donors were artificially infected with 5 or 100 CFU of organisms commonly causing bacteremia. Positive results by SIFT-MS analysis of headspace gases were recorded for 53/60 (88.3%) bottles at 8h, and 58/60 (96.6%) bottles at 24 h. We conclude that SIFT-MS is a sensitive method for the detection of microbial VOCs. Furthermore, profiles of the VOCs detected may allow simultaneous identification of infecting organisms. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:628 / 631
页数:4
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